idc 10030 lab malaria/microbiology
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idc10030 on April 27, 2011
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idc 10030 lab malaria/microbiology
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49 terms
Terms | Definitions |
|---|---|
 what part of the cell encompasses cytoplasm? | cell wall |
| what part of the cell is the material that holds organelles? | cytoplasm |
| what part of the cell is for motility? | flagella |
| the vibrio cholerae has what type of flagella? | lopotrichous - bunch of flagella at one end |
| the spirillum serpens has what type of flagella? | amphitrichous-bunches of flagella at both ends |
| the e coli has what type of flagella? | peritrichous-entire surface covered with flagella or cilia |
| what type of the cell attaches to other cells? | pilli |
| which type of bacterial cell morphology lacks a distinct shape? | pleomorphic |
| if you see gram positive chains in your micro exam, what is the specific bacteria? | streptococci |
| what type of toxins act on gi tract causing diarrhea? | enterotoxins |
| what bacteria species causes neurotoxins? | exotoxins |
| if you suspect uti caused by ecoli, what urine collection method do you use? | clean catch |
| what are examples of neurotoxins that act on the nervous system? | tetanus and botulism |
| why do you not use outside skin samples for specimen collection? | due to the high number of normal flora bacteria |
| which reagent if the primary stain and what color does it stain? | crystal violet: blue/purple |
| Which reagent is the mordant, is capable of intensifying or deepening the reaction of a specimen to a stain and fixes a dye in or on substances? | grams iodine |
| which reagent is the decolorizer? | ethanol and acetone solution 50/50 |
| which reagent is counterstain and what color does it stain? | safranin-gives a red pink color |
| which gram stain will stain dark blue to purple/black? | gram positive |
| which gram stain will stain deep pink to red? | gram negative |
| what is the purpose of the grams stain? | a differential staining technique vital to the CHARACTERIZATION AND CLASSIFICATION of many types of bacteria |
| what is gram positive lancet shaped diplococci that is the causative agent of pneumococcal pna? | streptococcus pna |
| what is a gram negative bacilli that maybe curve shaped and is motile with single flagellum? | vibrio cholerae |
| what is a gram negative bacilli that occurs in singles, pairs, short and long chains? | klebsiella pna |
| what is a gram positive that looks like "watermelon seeds", will often have budding, may often have mickey mouse shape and branching (false hyphae) that appear as road maps? | yeast |
| what is a gram positive bacilli that may show irregular staining granules showing a "beaded" appearance and have a characteristic chinese letter or as L, V, or Y shapes? | corynebacterium diptheria |
| what is a parasitic infxn caused by the intro of the protozoan organisms into the blood by the bite of a female anopheles mosquito? | malaria |
| sporozoites enter parenchymal cells of what organ to become shizonts? | liver |
| what is the life cycle of plasmodium? (malaria parasite) | female anopheles mosquito bites man sporozoites injected into man when bitten, sporozoites enter parenchymal cells of liver to become schizonts, schizonts rupture releasing merozoites and invade RBC,s merozoites then develop into mature schizontsl |
| all stages of asexual cycle can be demonstrated in a peripheral blood smear with the exception of what? | p. falciparum |
| what gametocyte is female-curved sausage/banana shaped with central large chromatin dot covered with centralized mass of hemazoin? | macrogametocyte |
| what gametocyte is male-shorter and wider than female with rounded ends, diffused chromatin seen with hemazoin granules? | microgametocyte |
| which type of malaria is most fatal and the malignant tertian is 48 hrs? | plasmodium falciparum |
| which type of malaria is most common where tertian is 48hrs? | plasmodium vivax |
| which type of malaria is the quartan 72hrs? | plasmodium malariae |
| which type of malaria is the ovale 48 hrs? | plasmodium ovale |
| what is the proper way to scan the malaria smear? | should be scan, at least 100 fields, then report |
| what is a multicellular heterotrophic organism occupying a seperate kingdom from the plant and animal kingdoms? | fungi |
| fungi has basic branching and intertwining structure called what? | mycelium |
| mycelium composed of tubular filaments called what? | hyphae |
| what is most common yeast isolate? | candida albicans |
| what tinea is also known as athlete's foot? | tinea pedis |
| what is defined as a fungal infection upon the skin? | dermatophytes (tinea spp) |
| what is the nutrient source for dermatocyte? | uses keratin as a nutrient source but are not capable of penetrating subcutaneous skin |
| how long do you allow koh to stand for? | 5 min. |
| what do you use to scrape skin from the active edge of the lesion on to a glass slide? | a #15 scalpel |
| when is the optimum time to collect a malaria smear? | immediately after onset of fever or before fever spike for best results |
| where do you send unstained malaria smears? | nepmu for id and confirmation |
| what bacteria is intra/extracellular diagnostic for? | gram negative diplococci (n.gonorrhea) |
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