Add 1 drop of red cell suspension from panel cells and 2 drops of patient serum , at room temp, spin ofr 15 seconds and read for agglutination and/or hemolysis - Name the phase
immediate spin phase
which phase detects IgM antibodies. commenly detected are Anti-H , Anti -IH and Anti-p?
Immediate spin phase
what does the immediate spin phase detect?
Anti-H, Anti-IH, Anti-p
The albumin /liss phase is detected at what temperature?
The albumin liss phase detects what antibodies?
some igG, some Rh system, some Anti-K, some anti-leA and anti-LeB, some MNS system
The AHG phase happens at what temperature ?
reactions are read _______________ for agglutination and or hemolysis.
reactions that appear macroscopically negative must be checked for what?
when shaking the tube you cannot read the reaction until what?
the cell button is dislodged
hemolytic antibodies are most often seen with what system of antibodies ?
Lewis or Kidd
what systems antibodies are capable of reacting at ALL phases and temps?
antibodies do not read__________ so they should not always act exactly like the books say they should.
what are other names to describe non-ABO antibodies ?
unexpected, atypical and irregular
antibody identification (id) is most often concerned with what?
non-ABO antibodies that may appear in a persons serum
The antibody panel is used to identify:____________&_____________
immune antibodies igg : pregnancy,transfusion & Naturally occuring (igM) : unknown stimulus
a history of what conditions can be directly related to the production of allo & auto antibodies?
history of pregnancy or transfusion, patient diagnosis, patient drug therapy , history of recent injection of RHLG
Results that indicate the presence of unexpected antibodies
H.I.P.S.T.D hemolytic disease of the newborn, incompatible crossmatch, positive direct anti-globulin test, serum with a positive antibody screen, transfusion reaction, discrepancy between abofront(cell) and reverse (serum) group.
define incompatible crossmatch
recipient serum antibody reacting with donor antigen
define the discrepancy between the ABO front (cell and reverse(serum) group
serum contains the antibody that is agglutinating with the A1 or B reverse grouping cells , or the antibody is reaction with an antigen other then A or B
define transfusion reaction
secondary response may cause transfusion reaction as antibody production increases
define hemolytic disease of the newborn
maternal antibodies causing the distruction of fetal or newborn red cells
positive direct antiglobulin test- define it
person producing an autoantibody
serum or plasma specimens may be used when?
with in 72 hours of drawing if refirgerated because antibodies degrade with in time
hemolysis is a positive reaction n most blood banking . true or false
serum must be collected in what kind of tube and why ?
a regular red top tube with no serum seperaters because they bind antibodies
antibody identification panel determines what ?
the specificty of the antibody
the antibody id panel is usually only done when what is detected?
in the presence of an atypical antibody
what is something that causes inaccurate test results?
rouleaux may resemble agglutination due to _______________________.
increased serum protein
rouleaux formation is seen in which phases of testing
saline and albumin but NOT AHG
how do you solve a rouleaux formation?
saline replacement technique
this consists of a persons own red cells and serum. this tube is ran through various temps and phases identical to the other tubes in the procedure..
the auto control is most often __________?
the auto control may be postive in certain situations. explain...
rouleaux, albumin agllutinating phenomena,anti-caprylate in a persons serum ,
panel results must be compared to the __________ that matches the panel
the lot number of the antigram must match the __________?
lot number of the panel cell vial
for a particular vial to be considered neagtive...?
the serum must be non-reactive in all phases
ANTIGENS present in the non-reactive vial are crossed of the antigram(ruled out ) since the corresponding __________ did not react
Antigen + Antigen=
Antigen + no antibdy =
after the rulling out process what do you do?
circle the remaining antigens
pattern of reactivity of the antibodies will coincide with what?
the pattern of the antgen as it appears on the antigram
if only one antigen remains and the patterns of reactivity match then what ?
the antibody has been identified!