Micr221 Final Exam Study 5 More Labs - TSIA

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Is triple sugar iron agar differential, selective, or enriching?

differential

TSIA contains which three carbohydrates, one at 0.1%, and two 1% concentrations?

glucose, lactose and sucrose

What two other compounds are in TSIA?

ferrous ions and phenol red

Which four things can be quantified/ noted from each tube? (alphab)

butt, gas, hydrogen sulfide, slant

Fermentation, an acid-producing reaction, will turn the tube which colour?

yellow

An alkaline reaction due to the aerobic utilisation of organic acids by enteric bacteria when fermentable subtrates have been used turns the tube what colour?

Deep red

If bacteria produce H2S (hydrogen sulfide) it will react with the ferrous ions to produce iron sulfide to form a what?

black precipitate

TSIA differentiates which types of bacteria?

gram-negative enteric bacilli

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