Transcription of this operon is maximally activated when lactose is present and glucose is present?
In what step is transcription decreased due to RNA secondary protein formation.
What type of cells is gene expression off unless specifically turned on by the binding of transcriptional activator protein due to heterochromatin.
A cis enhancer is
What is the transcriptional activating domain responsible for?
Transcriptional activating domain is responsible for activation of a transcription factor.
The constellation of biochemical modifications to histone tails that dictates a particular chromatin state
DNA methylation of nitrogenous bases
What happens to DNA to cause it to be chemically marked?
Associated with transcriptional activation
How do restriction enzymes work?
Restriction enzymes break apart DNA molecules by breaking covalent bonds
What allows for insertion of a DNA fragment into a vector
Multiple clone site
Is not used as a method of DNA transfer used to get a recombinant DNA into a E. coli host
Required to convert a mRNA transcript to cDNA
What is the Blue White Screen used for?
Enables you to select a bacterial cell containing a vector with your DNA insert
Which RNA molecules are involved in post-transcriptional gene splicing
miRNA and siRNA
Transcribed double stranded RNA
RNA polymerase II
Not involved in miRNA processes
Cis acting DNA regulatory elements
A site on a DNA molecule that functions as a binding site for a sequence-specific DNA binding protein. The protein binging to this site affects only nearby DNA sequences on the same molecule
Trans acting factors
A diffusible regulatory protein that binds to a specific cis-acting element
Pattern of modification of the histone tails that may carry information required for correct chromatin assembly
Functional RNA that regulates the amount of protein produced by a eukaryotic gene
Adding ubuitin chains to a protein targeted for degradation
Post translational modifications
Where a gene inherited form one of the parents is not expressed, even though both gene copies are functional. Imprinted genes are methylated and inactivated in the formation of male or female gametes.
The process that produces a gene or a chromosome set differing from that of the wild type
Single nucleotide change that still produces same amino acid
Causes the translation of a different mutation
Produces a stop codon which causes early termination
Goes from a purine to a purine
Goes from a purine to a pyridamine
Removes codon sequence
Inserts codon sequence
Flips a codon sequence
Relocation to a different part of the genome
A genome having a chromosome number that differs from the normal chromosome number for the species by a small number of chromosomes
A cell/organism having three or more chromosome sets
Unequal crossing over
Site specific recombination
How do x-rays and UV light produce mutation?
How does the process of DNA repair work?
Intermediate between DNA and synthesis of proteins
Components of ribosomes that guide the amino acid chains made by tRNA and mRNA
Bring the correct amino acis to mRNA in translation
Removes introns from mRNA transcripts
Regulate the amount of protein produced by many eukaryotic genes
Short double-stranded RNA prevents the spread of viruses and transposable elements