Advertisement Upgrade to remove ads

alolimmunity

comes from someone else

HDN description

mom is alloimmunized from baby's RBCs and mom makes antibodies to baby RBCs and starts lysing the baby cells.
-results in erythroblastosis fatalis

IgG antibodies

D,K,Rh,K,Ss

other than ABO& RH, the Ab that causes HDN the most is

ABO and Rh, then Kell is next

when prenatal testing detects mom antibodies, what do you need to do next

an antibody ID panel

If IgM is detected on an antibody ID panel and IgM is detected, is that clinically significant?

no- only if IgG is detected

what test is used to assess fetal maternal bleeds

rosette test

if a babies Rh positive blood mixes with a mothers Rh negative blood how do you remove the baby's cells?

add anti-D to mom's cells and rosettes will form (qualitative). Because the anti-D will bind with the baby cells.

Kleiheiuer-Betke test facts

-quantitative
-stains hgb F
-uses acid buffer on slide
-can count hgb F cells
moms hgb A shows as ghost cell

if a pregnant woman is injured and intrauterine bleeding occurs, what needs done?

-intrauterine transfusion
-O neg blood less than 7 days old
-CMV neg blood
-blood neg for hgb's

How do you prevent HDN?

Use rogam 72 hours after delivery if baby is RH pos (1st baby)
for second pregnancy, rogam at 28 weeks and after birth if baby is Rh pos

how do you determine if there is an internal bleed in a pregnant woman?

draw mom's blood and perform a rosette test

KB test: what specimen is NOT suitable for testing fetal-maternal hemorrhage

cord blood
(used for QC purposes only)

KB test: maternal specimen storage

2-8°C for up to 2 weeks

KB test: what is used for QC

EDTA male blood or cord blood

KB test: do not do what to slides

refix

KB test: slide storage

in freezer for several weeks but use at room temp

KB test: stain

citrate-phosphate buffer

KB test: slides to use

positive and negative control

KB test: what stains hot pink

-fetal cells
-lymphocytes (nucleated or granulocytic)
-platelets (small)

KB test: adult cell morphology

ghost cells

KB test: result

percentage of fetal to adult counted cells from slide

KB test: fetal hgb is:http://quizlet.com/create_set/#add-row

acid resistant

KB test: adult hgb is:

dissolved from cells because is is not acid resistant

most common cause of HDN

Anti-D
Anti K
Anti C
Anti Fy

KB test: used for what

do determine how much rogham needs to be given

2 reasons to do cord blood testing:

1. if mom is O+
2. if mom is Rh neg

after giving birth to an Rh positive infant, a mom who previously tested Rh negative may test weak D pos. Why?

baby's blood crossed over into mom's blood and she developed antibodies

a mother has an anti-D and an infant who has HDFN symptoms. the infant tests are Rh neg. what may cuase a false neg Rh test in the infant?

Mom's blood crossed over into baby's blood so mom's antibodies are being detected in baby

SOP

most important lab document

TJC

college of american pathologists the joint commission

CAP

college of american pathologists

QC reagents how often

every day and when you open a new bottle

middleware

software that converts instrument info into hospital readable data

LIS

lab info system

LIS benefits

-reduced paperwork
-store data
-immediate access to data
-barcodes reduce misidentification
-good reports from data

minimum requirements of lab instruments

-access code for security
-unique accession numbers
-unique file for each assay
-record date, time and collector
-store and print bar codes
-interface with current system

the most important thing to do when solving a problem

document

RBC life time

120 days

hemolytic anemia=

decreased RBC survival

warm AIHA reaction

-IgG
-37°C
-DAT positive= adsorption and elution

cold AIHA reaction

-IgM
-PCH- auto anti-P and donath landsteiner Ab
-DAT pos due to C3d
-adsorption may be necessary

drug induced reaction

-increase doses of IV penicillin
-second generation cefalosporin
-stop drug= stop reaction

AABB

american association of blood banks

what do you do to get AIHA to away

prewarm specimen

elution

removing Abs that are bound to RBCs

adsorption

add specific Ag to remove Ab from serum

Why do we elute serum

so Ags in serum won't get in the way of tests. We can get it out of the way and continue searching for what we are looking for.

DAT (direct antiglobulin test) does what

tests for in vivo coating of RBCs with Ab

pos DAT can tell us:

-if pt has been transfused, they may have an alloantibody coating the transfused cells
-if pt hasn't been transfused, they may have an autoantibody coating their own cells

what causes paroxismal cold hemoglobinurea

donath landsteiner

FDA

regulates drugs and devices

CDC

makes safety and disease recommendations to OSHA

EPA

environmental protection agency- assesses medical waste

DOT

all transportation except mail

TJC

accrediting agency for hospitals

AABB

accredits bloodbanks

CAP

lab peer review

HCFA

health care financial administration
-determines if a facility can get medicare reimbursement

root cause analysis

why somethin's not working (check for error systematically)

accuracy

correct

precision

re-creatable but not necessarily correct

validation

standardizing - establish that a process produces a specific result

calibration

standardizing to a known value

quality control

assesses precision and accuracy

proficiency test

assesses proficiency of techs, instruments and tests (COLA,CAP)

competency assessment

know that techs are capable and skillful

preventative maintenance

equipment care to maximize it's duration

change control

old procedures to new ones

Please allow access to your computer’s microphone to use Voice Recording.

Having trouble? Click here for help.

We can’t access your microphone!

Click the icon above to update your browser permissions above and try again

Example:

Reload the page to try again!

Reload

Press Cmd-0 to reset your zoom

Press Ctrl-0 to reset your zoom

It looks like your browser might be zoomed in or out. Your browser needs to be zoomed to a normal size to record audio.

Please upgrade Flash or install Chrome
to use Voice Recording.

For more help, see our troubleshooting page.

Your microphone is muted

For help fixing this issue, see this FAQ.

Star this term

You can study starred terms together

NEW! Voice Recording

Create Set