GRAM STAINING/CULTURING BACTERIA
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38 terms
Terms | Definitions |
|---|---|
 COUNTERSTAIN/ "SAFRANIN/ SECONDARY STAIN | Stain with a contrasting color to the primary stain. |
| GRAM POSITIVE bacteria | -Single thick cell wall -will stain PURPLE. |
| GRAM NEGATIVE bacteria | -Multiple thin layer walls. -Will stain RED/ PINK. |
| CRYSTAL VIOLET (PRIMARY STAIN) | will stain all gram positive microorganisms a PURPLE color. |
| SECONDARY STAIN "SAFRANIN" | Stains all gram negative microorganisms a bright PINK or RED color. |
| 4 | Gram's stain procedure requires how many solutions? |
| 2 | How many stains? |
| Always observe appropriate safety and health precautions. | What should always be done when working with infectious materials? |
| Flood the slide with the decolorizer until no violet color washes off. | After over laying the slide with Gram's iodine, let stand for a minute, hold for 45 degrees and rinsed with water, what is the next step? |
| 30-60 seconds | How long do you overlay the Safranin on the slide for? |
| Culture medium | Nutrient material prepared for growing microorganisms in the lab. |
| Plating"Culturing | The CULTIVATION of bacteria on plates |
| To understand the EFFECTS OF ANTIBIOTICS on the organism and indicate the susceptibility of the bacteria to concentrations of antibiotics. | What is the purpose of culture and sensitivity? |
| Adequate amounts of SALT and WATER | What essential nutrients in proper concentration are in a CULTURE MEDIUM? |
| Broth | Pure cultures cannot be obtained from this medium? |
| -Butts -Slants -Petri plates | Pertaining to Solid Agars; Solids are called? |
| PROTEINS | What provides a source of heat and energy? |
| Moisture | -Provides enough water to maintain the organism's life functions -Purified water recommended, either distilled or deionized. |
| BLOOD | Usually supplied in 3% PACKED cells or 5% whole blood. |
| Sheep's Blood | What is the most commonly used blood, but human blood can be used? |
| SUGAR(Carbohydrates) | Important in energy production. |
| Dyes, Bile salts, Sodium chloride, and Misc. inhibitors. | What are four types of culture substance inhibitors? |
| -Sodium -Sulfate -Sodium selenite -Antibiotics | Gram positive inhibitors? |
| -Phenol Red -Methyl Red -O Blue | List Culture substance indicators? |
| Trypticase soy broth (TSB) | Accelerates bacterial growth to a log rate in 6-8 hours |
| Mueller Hinton agar | -Used as a base for blood agar formulations. -Composed of beef infusion and startch. |
| Brain-Heart Infusion media (BI) | -Broth used to accelerate log growth. |
| Blood agar plates (BAP) | -Used to provide primary isolation of most bacteria. -Provides for differentiation of hemolysis. |
| Thayer-Martin agar (TM) | Used as selective media for Neisseria Sp. |
| Mannitol Salt agar (MSA) | Selective for gram positive organisms, especially Staphylococcus. |
| MacConkey (MAC) | Fermenters will produce red-pink COLONIES. |
| Eosin-Methlyene Blue | -Listed as slightly selective media for gram negative organisms. -Fermenters will show a green metallic sheen. |
| Hektoen-Enteric agar (HE) | -Designed for the recovery of Salmonella and Shigella. -Moderately selective for gram negative organisms. -Fermenters will produce BLACK percipitate. |
| Top | Where is the growth of an Aerobic bacteria located? |
| Bottom | Where is the growth of an Anaerobic Bacteria located? |
| Middle/ Mid-media growth | where is the growth of a Microaerophillic bacteria located? |
| Growth all over tube | Where is the growth of a Faculatative anaerobic bacteria located? |
| "Mat Method" | Aka Streak Plate Method. |
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