GRAM STAINING/CULTURING BACTERIA
|COUNTERSTAIN/ "SAFRANIN/ SECONDARY STAIN||Stain with a contrasting color to the primary stain.|
|GRAM POSITIVE bacteria|| -Single thick cell wall|
-will stain PURPLE.
|GRAM NEGATIVE bacteria|| -Multiple thin layer walls.|
-Will stain RED/ PINK.
|CRYSTAL VIOLET (PRIMARY STAIN)||will stain all gram positive microorganisms a PURPLE color.|
|SECONDARY STAIN "SAFRANIN"||Stains all gram negative microorganisms a bright PINK or RED color.|
|4||Gram's stain procedure requires how many solutions?|
|2||How many stains?|
|Always observe appropriate safety and health precautions.||What should always be done when working with infectious materials?|
|Flood the slide with the decolorizer until no violet color washes off.||After over laying the slide with Gram's iodine, let stand for a minute, hold for 45 degrees and rinsed with water, what is the next step?|
|30-60 seconds||How long do you overlay the Safranin on the slide for?|
|Culture medium||Nutrient material prepared for growing microorganisms in the lab.|
|Plating"Culturing||The CULTIVATION of bacteria on plates|
|To understand the EFFECTS OF ANTIBIOTICS on the organism and indicate the susceptibility of the bacteria to concentrations of antibiotics.||What is the purpose of culture and sensitivity?|
|Adequate amounts of SALT and WATER||What essential nutrients in proper concentration are in a CULTURE MEDIUM?|
|Broth||Pure cultures cannot be obtained from this medium?|
|Pertaining to Solid Agars; Solids are called?|
|PROTEINS||What provides a source of heat and energy?|
|Moisture|| -Provides enough water to maintain the organism's life functions|
-Purified water recommended, either distilled or deionized.
|BLOOD||Usually supplied in 3% PACKED cells or 5% whole blood.|
|Sheep's Blood||What is the most commonly used blood, but human blood can be used?|
|SUGAR(Carbohydrates)||Important in energy production.|
|Dyes, Bile salts, Sodium chloride, and Misc. inhibitors.||What are four types of culture substance inhibitors?|
|Gram positive inhibitors?|
| -Phenol Red|
|List Culture substance indicators?|
|Trypticase soy broth (TSB)||Accelerates bacterial growth to a log rate in 6-8 hours|
|Mueller Hinton agar|| -Used as a base for blood agar formulations.|
-Composed of beef infusion and startch.
|Brain-Heart Infusion media (BI)||-Broth used to accelerate log growth.|
|Blood agar plates (BAP)|| -Used to provide primary isolation of most bacteria.|
-Provides for differentiation of hemolysis.
|Thayer-Martin agar (TM)||Used as selective media for Neisseria Sp.|
|Mannitol Salt agar (MSA)||Selective for gram positive organisms, especially Staphylococcus.|
|MacConkey (MAC)||Fermenters will produce red-pink COLONIES.|
|Eosin-Methlyene Blue|| -Listed as slightly selective media for gram negative organisms.|
-Fermenters will show a green metallic sheen.
|Hektoen-Enteric agar (HE)|| -Designed for the recovery of Salmonella and Shigella.|
-Moderately selective for gram negative organisms.
-Fermenters will produce BLACK percipitate.
|Top||Where is the growth of an Aerobic bacteria located?|
|Bottom||Where is the growth of an Anaerobic Bacteria located?|
|Middle/ Mid-media growth||where is the growth of a Microaerophillic bacteria located?|
|Growth all over tube||Where is the growth of a Faculatative anaerobic bacteria located?|
|"Mat Method"||Aka Streak Plate Method.|