Phlebotomy Tubes for NHA Exam

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Lavendar Top Tube Additive

Contains the anticoagulant ethylenediaminetetraacetic acide (EDTA). EDTA inhibits coagulation by binding to the calcium present in the specimen. Tubes must be filled at least 2/3 full and inverted 8x.

Lavendar Top Tube Tests

CBC, H&H, ESR, Sickle Cell Screening

Light-Blue Top Tube Additive

Contains the anticoagulant Sodium Citrate, which prevents coagulation by binding to the calcium in the specimen. Sodium Citrate preserves the coagulation factors. Must be completely filled and inverted 3-4x

Light-Blue Top Tests

PT, APTT (or PTT), Fibrinogen Degradation Products (FDP), Thrombin Time (TT), Factor assays, Bleeding Time (BT)

Green Top Tube Additive

Contains anticoagulant Heparin combined with sodium, lithium, or ammoniumion. Heparin works by inhibiting thrombin in the coagulation cascade. It is not used for hematology because heparin interes with the Wright's stained blood smear. Invert 8x

Gray Top Tube Additive

Contains additives and anticoagulants. All gray top tubes contain glucose preservative. Invert 8x

Gray Top Tube Test

Fasting blood sugar (FBS), glucose tolerance test (GTT), blood alcohol levels, Lactic acid measurements

Red/Gray (speckled) Top Tube Additive

Also called tiger top and serum separator tubes (SST). Contain clot activators: glass particles, silica, and celite and thixotropic gel a serum separator. Invert 5x

Red/Gray (speckled) Top Tube Test

Most chemistry tests

Red Top Tube Additive

aka plain vacuum tube. Contains NO additive or anticoagulant. No need to invert.

Red Top Tube Test

Serum chemistry test, serology tests, blood bank

Yellow Top Tube Additive

Contains the anticoagulant sodium polyanetholesulfonate (SPS). Used to collect specimens to be cultured. Must used strict sterile technique Invert 8x

Pink Top Tube Additive

Potassium EDTA.

Pink Top Tube Test

Blood bank, immunohematology

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