-Requires oxygen to multiply
- Growth just on top
- Only aerobic growth
EX: Alcaligenes Faecalis
-Can multiply with or without oxygen, but grow prefereably with oxygen
- Growth everywhere, sometimes little on top
- Both aerobic and anerobic growth
EX: Eschecheria Coli
-Cannot multiply in presence of oxygen
- Growth:Gap from surface, only where there is no oxygen
- Only anerobic growth
EX: Clostridium Sporaogeneus
- Does not use oxygen and it is not affected by it
- Growth occurs evenly
- Only anaerobic growth
- Oxgen required but ONLY in low concentration
- Growth only where low concentration of oxygen has diffused into medium
- Only Aerobic growth
Contains chemicals that combine with oxygen rendering the medium suitable for the growth of anearobes. Because anaerobes might be killed by exposure to oxygen.
E. Coli in Anaerobe enviorment
Enzyme catalase is NOT PRODUCED
E. Coli in Aerobic enviorment
Enzyme catalase IS PRODUCED
What color does the oxygen indicator turn if oxygen is present
What color does the oxygen indicator turn when Oxygen is not present?
What is the purpose of Sodium thioglycate?
Removes oxygen from culture medium which mean it scavenges oxygen from the medium
Brewer anaerobic jar
Oxygen is exluded by adding a GAs Pak and palladium catalyst to combine the hydrogen with oxygen to form water.
What is the disadvantage of the Brewer jar?
1.You have to open the jar to view which wil introduce oxygen.
What does Resaruzin or Methylyne Blue indicate?
A dye that is used to indicate the prescnce of excess oxygen (PINK). White when oxygen is reduced.