Microbiology Lab Quiz
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44 terms
Terms | Definitions |
|---|---|
 Parfocal | ability of microscope to stay in focus form one objective to another |
| Total magnification | ocular x objective |
| resolution | ability of the microscope to distinguish b/w tow separate points |
| wet mount | slide preparation of liquid suspension covered with a coverslip |
| simple stain | staining that uses one stain |
| prokaryotic | bacteria no true nucleus smaller than eukaryote less comples unicellular |
| eukaryotic | protozoa, fungus, animals nucleus membrane bound organelles unicellular or multicellular |
| What is the function of a condenser? | condenses light to form a solid beam that focuses on specimen |
| What is the fuction of the ocular lens? | magnifies 10 x |
| What is the function of the objective lens? | magnifies 4x, 10x, 40x or 100x |
| What is the function of the diaphragm? | controls light to condenser |
| Is the protozoon euglena a prokaryote or eukaryote? | eukaryote |
| Is a bactium a prokaryote or eukaryote? | prokaryote |
| List 2 differences b/w a wet mount and a simple stain | -wet mount uses a slide cover and we view something alive -simple stains use an oil and we kill everything with heat fix |
| aseptic technique | -procedure done to reduce contamination -ex. flaming a loop, washing hands, keeping the lid on an agar plate |
| differential stain | -staining procedures that uses more than one stain -ex. gram stain |
| streak plate | -method using sample of bacteria to be spread out over a surface -goal: isolate colonies |
| Gram Stain Reactions Positive: Negitive: | Positive: purple Negitive: pink |
| Bacterial Arrangements: chains: clusters: pairs: random: | chains: cocci or bacilli clusters:cocci only pairs: cocci only random: bacilli only |
| Bacillus Gram reaction: Shape/ Morphology: Arrangement: | Gram reaction: positive Shape/ Morphology: rods Arrangement: chain |
| E. coli Gram reaction: Shape/ Morphology: Arrangement: | Gram reaction: negitive Shape/ Morphology: rods Arrangement: random |
| Staphylococcus Gram reaction: Shape/ Morphology: Arrangement: | Gram reaction: positive Shape/ Morphology: cocci Arrangement: clusters |
| Complex agar | -non selective media that contains a variety of ingredientswith no set formula -Base for most agars -Contains nutrients to grow most bacteria -Ex. Nutrient agar (NA), Tryptic Soy agar (TSA) |
| Enriched Agar | Complex agar enriched with other ingredients Ex. BAP |
| Selective Agar | -Complex agar with an added ingredient to inhibit one type of organism while another type grows -Ex. MAC (Selective for gram negative rods), MSA Selective for gpc (Staphylococcus species) |
| Differential Agar | -BAP can differentiate between bacteria that can hemolyze the red cells (break the red cells) -Causes a clearing around the colony |
| TSA will grow: | gram positive & gram negitive bacteria |
| NA will grow: | gram positive & gram negitive bacteria |
| BAP will grow: | gram positive & gram negitive bacteria |
| MAC will grow | gram negitive bacteria |
| Lactose fermenters will grow: | pink colonies |
| MSA will grow | gram positive bacteria |
| Mannitol fermenters will grow: | yellow colonies |
| Metabolism | sum total of all reactions in the cell |
| Strict aerobe | organisms that require oxygen in order to grow. |
| Facultative anaerobe | organisms that use aerobic respiration when oxygen is present and fermentation when oxygen is absent. These organisms grow with and without oxygen. |
| Strict anaerobe | organisms that cannot grow in the presence of oxygen |
| Catabolism | process of breaking down large molecules into smaller units that can be used by the organism. Energy is released in the process. |
| Fermentation | metabolic reactions that convert energy contained in nutrients into ATP without oxygen |
| What environment is created when the mineral oil is added? | anaerobic |
| Is Pseudomonas an oxidizer and/or fermenter? What type of bacteria is it? | oxidizer strict aerobic |
| Is E. coli an oxidizer and/or fermenter? What type of bacteria is it? | oxidizer and fermenter facultative anaerobic |
| What is the jar called that produces an anaerobic environment? | Gas- Pack anaerobic jar |
| What type of bacteria can grow in an anaerobic environment? | strict anaerobe and facilitative anaerobe |
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