Isolating and Separating DNA - Topic 6
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14 terms
Terms | Definitions |
|---|---|
 Gel Electrophoresis | Way to separate negatively charged DNA by size. |
| Ethidium Bromide | Binds to DNA and gives off orange/pink color under UV light |
| Electron Microscopy | Using electron beams to create microscopic images |
| Agarose | Gel used in electrophoresis |
| SDS-PAGE | Process whereby proteins are denatured and have a uniform negative charge. Separates only by size |
| 2D gel electrophoresis | Way to separate DNA segments by size using charge |
| What are the basic principles of DNA and RNA isolation? | Cell must be opened DNA must be extracted without sheering or damage - includes protecting from DNase. |
| How do you break open a cell to extract DNA? | Boiling, freezing, or mechanical disruption; Alkaline Lysis; Enzymatic Lysis |
| Describe Alkaline Lysis | Buffer created of SDS and a strong base used to disrupt cells. Treatment followed by neutralization with an acid |
| Describe Enzymatic Lysis | Enzymes used to disrupt cells |
| Lysozyme | Used to disrupt bacterial cell walls |
| Zymolase | Used to disrupt fungal cell walls |
| Proteinase k | Used to break open a cell. Also protects the DNA from DNase |
| How does electron microscopy work? | uses electromagnetic lens to focus a beam at a sample to produce an image of a microscopic object. |
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