Way to separate negatively charged DNA by size.
Binds to DNA and gives off orange/pink color under UV light
Using electron beams to create microscopic images
Gel used in electrophoresis
Process whereby proteins are denatured and have a uniform negative charge. Separates only by size
2D gel electrophoresis
Way to separate DNA segments by size using charge
What are the basic principles of DNA and RNA isolation?
Cell must be opened
DNA must be extracted without sheering or damage - includes protecting from DNase.
How do you break open a cell to extract DNA?
Boiling, freezing, or mechanical disruption; Alkaline Lysis; Enzymatic Lysis
Describe Alkaline Lysis
Buffer created of SDS and a strong base used to disrupt cells. Treatment followed by neutralization with an acid
Describe Enzymatic Lysis
Enzymes used to disrupt cells
Used to disrupt bacterial cell walls
Used to disrupt fungal cell walls
Used to break open a cell. Also protects the DNA from DNase
How does electron microscopy work?
uses electromagnetic lens to focus a beam at a sample to produce an image of a microscopic object.