Microbiology Test 1 (PPT 6)

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Zach0918  on September 24, 2011

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Microbiology Test 1 (PPT 6)

Electron Donor
Loses electron (oxidation) which is taken up by an electron acceptor (reduction)
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Electron Donor Loses electron (oxidation) which is taken up by an electron acceptor (reduction)
Electron Acceptor A substance that can accept electrons from an electron donor, becoming reduced in the process
Chemoheterotroph - energy from organic compounds - carbon from organic compounds - all animals, many bacteria
Chemoautotroph - energy from inorganic compounds
- carbon from CO2 or other inorganic compounds
Photoautotroph - energy from sunlight  carbon from CO2
- Cyanobacteria, plants
Photoheterotroph - energy from light
- carbon from organic compounds
Oxidative Phosphorylation - Synthesis of ATP coupled with electron transport!
- Most efficient method of generating ATP
- All animals, many bacteria!
Proton Motive Force ..., The potential energy stored in the form of an electrochemical gradient, generated by the pumping of hydrogen ions across biological membranes during chemiosmosis.
How does fermentation differ from respiration?Respiration: the physical and chemical processes by which an organism supplies its cells and tissues with the oxygen needed for metabolism and relieves them of the carbon dioxide formed in energy-producing reactions.

Fermentation: an enzymatically controlled anaerobic breakdown of an energy-rich compound (as a carbohydrate to carbon dioxide and alcohol or to an organic acid).
Give two examples of end products of fermentation. - Lactic acid
- Alcohol
- Other acids (ex: ace;c)
- Acetone
Compare and contrast aerobic respiration and anaerobic respiration. Aerobic:
• Uses pyruvate from
glycolysis
• Generates ATP
• Terminal electron acceptor is oxygen

Anaerobic:
• Glycolysis into pyruvate
• Modified Kreb's and ETS
• No O2 necessary!
• Terminal electron acceptor
- Nitrogen, iron, sulfate!
NAD+ becomes reduced to NADH during glycolysis and the Kreb's cycle. What happens to these NADH molecules in an organism using aerobic respiration? how is this different from what happens in a fermentation pathway?...
What are the end products of glycolysis? 2 Pyruvate molecules
What does the pentose phosphate pathway generate in a cell? The building blocks for DNA, RNA,
photosynthesis
Can glucose be metabolized via the Entner-Doudoroff pathway? Does this happen in eukaryotic cells, prokaryotic cells or both? Yes it is an alternative to glycolysis and yields less ATP. Catabolizes glucose to pyruvate with different enzymes then EMP pathway. Only found in prokaryotes. Net yield: 1 ATP, 1 NADH, and 1 NADPH per Glucose
What molecule enters the Kreb's cycle? what end products are produced?...
What is the function of the electron transport chain? where would you find this system in a bacterial cell? in a yeast cell? in one of your cells?...
Protease any enzyme that catalyzes the splitting of proteins into smaller peptide fractions and amino acids by a process known as proteolysis
Lipase an enzyme secreted in the digestive tract that catalyzes the breakdown of fats into individual fatty acids that can be absorbed into the bloodstream

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