BIOL 210 Lab Exam 1

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mandercruso  on September 26, 2011

Subjects:

microbiology

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BIOL 210 Lab Exam 1

1
BSL level not known to cause disease in healthy individuals
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1 BSL level not known to cause disease in healthy individuals
2 BSL level can cause disease in healthy people, but organisms are easily contained
3 BSL level can cause severe disease, especially when inhaled
4 BSL level highly virulent microbes posing extreme risk to humans, especially when inhaled
R = 0.61*wavelength/NA resolution formula
active feeding form define trophozoite
inactive form define cyst
photosynthetic define autotrophic
require organic carbon define heterotrophic
yeasts single-celled fungi
molds when fungi grow as strings of long, filamentous cells
hypha single filament of fungi
septate hypha is separated by walls
nonseptate hypha is continuous
dimorphic capable of growing yeasts or molds
sporangiospores spores of fungi that are contained in saclike container
conidia spores that are not enclosed within a spore-bearing sac but rather form from a special fertile hyphae
sterile free from all living bacteria, microorganisms and their spores
pathogen bacteria, virus, or other microorganism that can cause disease
inoculation vaccine as a precaution to prevent disease
loop inoculate broth using
needle inoculate deep broth using
loop or needle inoculate slant using
loop or needle inoculate agar using
pure culture contains a single kind of organism
framework arm and base of microscope
lamp base of microscope with light source
diaphragm adjustable disc with hole in center, located under the condenser of microscope
condenser beneath the stage of microscope; collects and focuses light on the specimen; keep at highest point
stage platform that holds slide of microscope
objective lens 3 or 4 lens above stage of microscope. 10x, 45x, 100x
ocular lens part of microscope that is closest to the eyes; 10x
focus adjustment 2 circle knobs; large = coarse, small = fine
tetrad 4 cocci cell group
sarcina 8 cocci cell group
staphylococcal irregular cluster of cells
negative stain stains the background dark but leaves the cells clear
simple stain stains the cell dark and leaves the background clear
india ink negative staining uses negative dyes such as
crystal violet and safranin basic (positive) dyes used for simple stains
differential stain allows the discrimination of one cell from another based on differential staining properties
crystal violet, iodine, ethyl alcohol, safranin gram staining steps
fixes a dye purpose of iodine
decolorizes purpose of ethyl alcohol
counterstain purpose of safranin
rod salmonella shape
coccus staphylococcus aureus shape
rod listeria innocua shape
rod bacillus megaterium shape
negative gram reaction salmonella typhimurium
positive gram reaction staphylococcus aureus
positive gram reaction listeria innocua
positive gram reaction bacillus megaterium
protozoa, unicellular, pseudopods amoeba characteristics
protozoa, autotrophic, 1 flagellum euglena characteristics
protozoa, heterotrophic, 1 flagellum Trypanosoma characteristics
protozoa, unicellular, heterotrophic, 2 flagella Trichomonas characteristics
protozoa, heterotrophic, unicellular, cilia Paramecium characteristics
protozoa, heterotrophic, unicellular, lack cell wall, non-motile, parasite, malaria Plasmodium characteristics
algae, autotrophic, unicellular, flagellated Chlamydomonas characteristics
fungus, unicellular, heterotrophic, budding, asexual and sexual yeast characteristics
fungus, heterotrophic, aseptate mycelium, sporangiospores, zygospores Rhizopus characteristics
fungus, septate mycelium, conidiospores, ascospores Aspergillus characteristics
fungus, multicellular, heterotrophic, septate mycelium, conidiospores, ascospores Penicillium characteristics
helminth, heterotrophic, multicellular, lack cell wall, flatworm, trematode Schistosoma characteristics
helminth, multicellular, lack cell wall, tapeworm Taenia characteristics
helminth, roundworm, cestode Trichinella characteristics

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