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5 Written questions

5 Matching questions

  1. Direct Agglutination reactions
  2. Immunoassays Multiple samples tested at once and has
  3. Hemagglutination
  4. ELISA is a example of immunoassay, indirect ELISA uses
  5. Monoclonal Antibody Production
  1. a red blood cells agglutinate
    certain viruses (influenza)
  2. b -using cells with antigen on them
    -naturally particulate antigens
  3. c Great sensitivity much greater than other tests, dependent on type of tag
  4. d -Immunization leads to Antibody-forming cells, which fuse with tumor cells to form hybridomas.
    -Hybidomas screened for antibody production and antibody-producing hybridomas cloned
    -Monoclonal antibodies isolated for cultivation
  5. e -antigen coated to plastic well
    -protein blocks remaining plastic surface
    -Serum added(primary antibody)
    -if antibodies(bind antigen)
    -if no antibodies(antigen not bound)
    -Indicator antibody(enzyme-linked anti-Ig antibody binds primary antibody)
    -Substrate(specific for enzyme linked to secondary antibody,enzyme causes substrate to change color)
    -Reactions(color change=
    antibody in serum,no color change=
    no antibody in serum)

5 Multiple choice questions

  1. Screen for antibodies (anti-HIV, anti-rubella)
  2. -coated spheres or cells
    -observe agglutination
  3. -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells
  4. marker(radioactive,fluorescent,enzyme)
  5. eliminate false negatives

5 True/False questions

  1. Agglutination reactions can occur with anti-lg-antibodiesparticles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs

          

  2. Qualitative agglutination-Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution

          

  3. The purpose of Immunofluorescence is to-Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica

          

  4. RPR - syphilis test (indirect, screening) many false positives-Ag is cardiolipin (beef liver)
    -specific test uses Ag from T. pallidum

          

  5. Single immunodiffusion-Two antigens and one antibody and place in separate wells
    Allow diffusion
    -Lines of precipitation
    if continuous = identical antigens
    if crossing lines =completely different antigens
    if continuous with spur= partial identity

          

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