NAME: ________________________

Micro 6 Test

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5 Written Questions

5 Matching Questions

  1. Single immunodiffusion
  2. Direct Agglutination reactions
  3. The purpose of Immunofluorescence is to
  4. Test issues (Sensitivity)
  5. RapidStrep is VERY specific, but not
  1. a -detect specific proteins in cells
    -detect viruses in cells
    -identify microbial cells
    -identify and sort cells
    -fluorescent activated cell sorter (FACS)
  2. b eliminate false negatives
  3. c too sensitive, lots of false negatives because Monoclonal Ab based
  4. d -Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen
  5. e -using cells with antigen on them
    -naturally particulate antigens

5 Multiple Choice Questions

  1. toxin, not bug
  2. -Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica
  3. Hormones (HCG, LH, HGH, FSH)
    Viral or bacterial antigens
  4. -results in mix soluble antigen and antibody
    -excess antigen or antibody--no precipitate
    -zone of equivalence--precipitate forms
  5. Great sensitivity much greater than other tests, dependent on type of tag

5 True/False Questions

  1. Qualitative agglutination-Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution


  2. In Gel precipitation-Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence


  3. Immunoassays Detect antigen or antibody and use a secondary antibody tagged with-Bind to cell
    -Visualize under UV light


  4. Indirect Agglutination reactions-using cells with antigen on them
    -naturally particulate antigens


  5. Zone of equivalencechange the amount of antigen with constant amount of antibody or the other way around


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