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5 Written Questions

5 Matching Questions

  1. Immunoassays Detect antigen or antibody and use a secondary antibody tagged with
  2. Immunoassays Multiple samples tested at once and has
  3. Indirect Ab detection of Fluorescence microscopy procedure
  4. Qualitative agglutination
  5. Complement fixation
  1. a marker(radioactive,fluorescent,enzyme)
  2. b -Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution
  3. c -known Ag (ie, Toxoplasma gondii) on slide
    -incubate with patient serum 25 min.
    -wash unbound serum away
    -incubate with conjugated anti-Hu gamma globulin ~25 min.
    -wash unbound Ab away
    detect in fluorescent microscope
  4. d Great sensitivity much greater than other tests, dependent on type of tag
  5. e Positive reaction:
    -Antibody present in serum
    -Serum added to test antigen
    -Bound antibody "fixes" complement
    -No available complement to lyse indicator cells
    Negative reaction
    -No antibody in serum
    -Complement not "fixed"
    -Free complement lyses indicator cells

5 Multiple Choice Questions

  1. red blood cells agglutinate
    certain viruses (influenza)
  2. Hormones (HCG, LH, HGH, FSH)
    Viral or bacterial antigens
  3. -coated spheres or cells
    -observe agglutination
  4. too sensitive, lots of false negatives because Monoclonal Ab based
  5. -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells

5 True/False Questions

  1. Test issues (Specificity)eliminate false negatives


  2. Advantages of immunodiagnostics-Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica


  3. Single immunodiffusion-Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen


  4. In Gel precipitation-Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence


  5. Types of precipitation reactions-using cells with antigen on them
    -naturally particulate antigens


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