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5 Written Questions

5 Matching Questions

  1. Hemagglutinin (HA), an antigenic glycoprotein from Influenza viruses morphology
  2. In Gel precipitation
  3. In Double immunodiffusion
  4. Nurses performing more
  5. Types of precipitation reactions
  1. a -Two antigens and one antibody and place in separate wells
    Allow diffusion
    -Lines of precipitation
    if continuous = identical antigens
    if crossing lines =completely different antigens
    if continuous with spur= partial identity
  2. b -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence
  3. c -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells
  4. d Test tube
    Capillary tube
    Agarose plate
  5. e POC testing than ever before because of the Immediate results

5 Multiple Choice Questions

  1. -Ag is cardiolipin (beef liver)
    -specific test uses Ag from T. pallidum
  2. red blood cells agglutinate
    certain viruses (influenza)
  3. -results in mix soluble antigen and antibody
    -excess antigen or antibody--no precipitate
    -zone of equivalence--precipitate forms
  4. -patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope
  5. -Immunization leads to Antibody-forming cells, which fuse with tumor cells to form hybridomas.
    -Hybidomas screened for antibody production and antibody-producing hybridomas cloned
    -Monoclonal antibodies isolated for cultivation

5 True/False Questions

  1. Test issues (Sensitivity)eliminate false positives


  2. Direct Agglutination reactions-using cells with antigen on them
    -naturally particulate antigens


  3. C. difficile test looks fortoxin, not bug


  4. Complement fixationred blood cells agglutinate
    certain viruses (influenza)


  5. Indirect Agglutination reactions-using cells with antigen on them
    -naturally particulate antigens


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