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5 Written questions

5 Matching questions

  1. In Double immunodiffusion
  2. Qualitative agglutination
  3. In Gel precipitation
  4. ELISA is a example of immunoassay, indirect ELISA uses
  5. Immunofluorescence use Antibody with fluorescent label and they
  1. a -Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution
  2. b -Two antigens and one antibody and place in separate wells
    Allow diffusion
    -Lines of precipitation
    if continuous = identical antigens
    if crossing lines =completely different antigens
    if continuous with spur= partial identity
  3. c -Bind to cell
    -Visualize under UV light
  4. d -antigen coated to plastic well
    -protein blocks remaining plastic surface
    -Serum added(primary antibody)
    -if antibodies(bind antigen)
    -if no antibodies(antigen not bound)
    -Indicator antibody(enzyme-linked anti-Ig antibody binds primary antibody)
    -Substrate(specific for enzyme linked to secondary antibody,enzyme causes substrate to change color)
    -Reactions(color change=
    antibody in serum,no color change=
    no antibody in serum)
  5. e -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence

5 Multiple choice questions

  1. -Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica
  2. -Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen
  3. red blood cells agglutinate
    certain viruses (influenza)
  4. Positive reaction:
    -Antibody present in serum
    -Serum added to test antigen
    -Bound antibody "fixes" complement
    -No available complement to lyse indicator cells
    Negative reaction
    -No antibody in serum
    -Complement not "fixed"
    -Free complement lyses indicator cells
  5. toxin, not bug

5 True/False questions

  1. Immunoassays Multiple samples tested at once and hasGreat sensitivity much greater than other tests, dependent on type of tag


  2. The purpose of Immunofluorescence is to-Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica


  3. Indirect Immunoassays isHormones (HCG, LH, HGH, FSH)
    Viral or bacterial antigens


  4. Test issues (Sensitivity)eliminate false negatives


  5. RAST - radioallergosorbentPOC testing than ever before because of the Immediate results


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