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Micro 6 Test

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5 Written Questions

5 Matching Questions

  1. The purpose of Immunofluorescence is to
  2. In Gel precipitation
  3. In Double immunodiffusion
  4. Types of precipitation reactions
  5. Nurses performing more
  1. a -Two antigens and one antibody and place in separate wells
    Allow diffusion
    -Lines of precipitation
    if continuous = identical antigens
    if crossing lines =completely different antigens
    if continuous with spur= partial identity
  2. b Test tube
    Capillary tube
    Agarose plate
  3. c POC testing than ever before because of the Immediate results
  4. d -detect specific proteins in cells
    -detect viruses in cells
    -identify microbial cells
    -identify and sort cells
    -fluorescent activated cell sorter (FACS)
  5. e -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence

5 Multiple Choice Questions

  1. -Ag is cardiolipin (beef liver)
    -specific test uses Ag from T. pallidum
  2. -Bind to cell
    -Visualize under UV light
  3. -coated spheres or cells
    -observe agglutination
  4. rat hepatocytes b/c liver cells have large nuclei
  5. red blood cells agglutinate
    certain viruses (influenza)

5 True/False Questions

  1. Direct Ag detection of Fluorescence microscopy procedure-patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope


  2. Hemagglutinin (HA), an antigenic glycoprotein from Influenza viruses morphologyparticles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs


  3. Indirect Immunoassays isHormones (HCG, LH, HGH, FSH)
    Viral or bacterial antigens


  4. Direct Immunoassays areScreen for antibodies (anti-HIV, anti-rubella)


  5. RAST - radioallergosorbent-indirect agglutination
    -measures IgE specific to a particular Ag
    -Ag-bead+pt serum  IgE-Ag-bead+anti-IgE-125I


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