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5 Written Questions

5 Matching Questions

  1. Direct Ag detection of Fluorescence microscopy procedure
  2. Immunofluorescence use Antibody with fluorescent label and they
  3. RPR - syphilis test (indirect, screening) many false positives
  4. Direct Immunoassays are
  5. In Gel precipitation
  1. a -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence
  2. b -Bind to cell
    -Visualize under UV light
  3. c -patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope
  4. d -Ag is cardiolipin (beef liver)
    -specific test uses Ag from T. pallidum
  5. e Hormones (HCG, LH, HGH, FSH)
    Drugs
    Viral or bacterial antigens

5 Multiple Choice Questions

  1. -Two antigens and one antibody and place in separate wells
    Allow diffusion
    -Lines of precipitation
    if continuous = identical antigens
    if crossing lines =completely different antigens
    if continuous with spur= partial identity
  2. eliminate false negatives
  3. -Similar to precipitation reaction
    -Visible reaction because antigen or antibody is on larger molecule (cell,latex bead,charcoal)
    -Interaction of antigen and antibody causes
    clumping of large particles
  4. Test tube
    Capillary tube
    Agarose plate
  5. -indirect agglutination
    -measures IgE specific to a particular Ag
    -Ag-bead+pt serum  IgE-Ag-bead+anti-IgE-125I

5 True/False Questions

  1. ELISA is a example of immunoassay, indirect ELISA usesScreen for antibodies (anti-HIV, anti-rubella)

          

  2. Qualitative agglutinationred blood cells agglutinate
    certain viruses (influenza)

          

  3. Test issues (Specificity)eliminate false positives

          

  4. Monoclonal Antibody Production-Similar to precipitation reaction
    -Visible reaction because antigen or antibody is on larger molecule (cell,latex bead,charcoal)
    -Interaction of antigen and antibody causes
    clumping of large particles

          

  5. Lupus - indirect test for anti-nuclear Ab (ANA) Uses-coated spheres or cells
    -observe agglutination

          

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