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5 Written Questions

5 Matching Questions

  1. Lupus - indirect test for anti-nuclear Ab (ANA) Uses
  2. C. difficile test looks for
  3. Advantages of immunodiagnostics
  4. In Gel precipitation
  5. Immunoassays Multiple samples tested at once and has
  1. a rat hepatocytes b/c liver cells have large nuclei
  2. b Great sensitivity much greater than other tests, dependent on type of tag
  3. c toxin, not bug
  4. d -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence
  5. e -Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica

5 Multiple Choice Questions

  1. -using cells with antigen on them
    -naturally particulate antigens
  2. -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells
  3. red blood cells agglutinate
    certain viruses (influenza)
  4. Hormones (HCG, LH, HGH, FSH)
    Drugs
    Viral or bacterial antigens
  5. POC testing than ever before because of the Immediate results

5 True/False Questions

  1. Direct Ag detection of Fluorescence microscopy procedure-known Ag (ie, Toxoplasma gondii) on slide
    -incubate with patient serum 25 min.
    -wash unbound serum away
    -incubate with conjugated anti-Hu gamma globulin ~25 min.
    -wash unbound Ab away
    detect in fluorescent microscope

          

  2. Indirect Ab detection of Fluorescence microscopy procedure-known Ag (ie, Toxoplasma gondii) on slide
    -incubate with patient serum 25 min.
    -wash unbound serum away
    -incubate with conjugated anti-Hu gamma globulin ~25 min.
    -wash unbound Ab away
    detect in fluorescent microscope

          

  3. RPR - syphilis test (indirect, screening) many false positives-Ag is cardiolipin (beef liver)
    -specific test uses Ag from T. pallidum

          

  4. Single immunodiffusion-Two antigens and one antibody and place in separate wells
    Allow diffusion
    -Lines of precipitation
    if continuous = identical antigens
    if crossing lines =completely different antigens
    if continuous with spur= partial identity

          

  5. Test issues (Specificity)eliminate false positives

          

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