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5 Written questions

5 Matching questions

  1. Direct Immunoassays are
  2. Visible soluble precipitate
  3. Immunofluorescence use Antibody with fluorescent label and they
  4. ELISA is a example of immunoassay, indirect ELISA uses
  5. Zone of equivalence
  1. a -Bind to cell
    -Visualize under UV light
  2. b Hormones (HCG, LH, HGH, FSH)
    Drugs
    Viral or bacterial antigens
  3. c -antigen coated to plastic well
    -protein blocks remaining plastic surface
    -Serum added(primary antibody)
    -if antibodies(bind antigen)
    -if no antibodies(antigen not bound)
    -Indicator antibody(enzyme-linked anti-Ig antibody binds primary antibody)
    -Substrate(specific for enzyme linked to secondary antibody,enzyme causes substrate to change color)
    -Reactions(color change=
    antibody in serum,no color change=
    no antibody in serum)
  4. d -results in mix soluble antigen and antibody
    -excess antigen or antibody--no precipitate
    -zone of equivalence--precipitate forms
  5. e change the amount of antigen with constant amount of antibody or the other way around

5 Multiple choice questions

  1. -trimeric protein
    -13 major antigenic classes
    -functions in viral attachment to a variety of host cells
  2. -indirect agglutination
    -measures IgE specific to a particular Ag
    -Ag-bead+pt serum  IgE-Ag-bead+anti-IgE-125I
  3. Test tube
    Capillary tube
    Agarose plate
  4. Positive reaction:
    -Antibody present in serum
    -Serum added to test antigen
    -Bound antibody "fixes" complement
    -No available complement to lyse indicator cells
    Negative reaction
    -No antibody in serum
    -Complement not "fixed"
    -Free complement lyses indicator cells
  5. -Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence

5 True/False questions

  1. The purpose of Immunofluorescence is to-detect specific proteins in cells
    -detect viruses in cells
    -identify microbial cells
    -identify and sort cells
    -fluorescent activated cell sorter (FACS)

          

  2. Lupus - indirect test for anti-nuclear Ab (ANA) Uses-coated spheres or cells
    -observe agglutination

          

  3. Nurses performing more-Agar dish is the solid medium
    -One well contains antibody
    -Other well contains antigen
    -Allow diffusion and form precipitate at zone of equivalence

          

  4. RapidStrep is VERY specific, but nottoo sensitive, lots of false negatives because Monoclonal Ab based

          

  5. C. difficile test looks forPositive reaction:
    -Antibody present in serum
    -Serum added to test antigen
    -Bound antibody "fixes" complement
    -No available complement to lyse indicator cells
    Negative reaction
    -No antibody in serum
    -Complement not "fixed"
    -Free complement lyses indicator cells

          

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