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5 Written questions

5 Matching questions

  1. Immunoassays Detect antigen or antibody and use a secondary antibody tagged with
  2. Advantages of immunodiagnostics
  3. RAST - radioallergosorbent
  4. C. difficile test looks for
  5. Agglutination reactions can occur with anti-lg-antibodies
  1. a -Faster!
    -More field- and POC-friendly
    -Built-in controls for better accuracy/interpretation
    -Entamoeba histolytica
  2. b marker(radioactive,fluorescent,enzyme)
  3. c -indirect agglutination
    -measures IgE specific to a particular Ag
    -Ag-bead+pt serum  IgE-Ag-bead+anti-IgE-125I
  4. d toxin, not bug
  5. e particles with attached antigens+ complementary antibodies to antigens> antibodies bind to antigens but they can't cause agglutination> when anti-lg antibodies added, agglutination occurs

5 Multiple choice questions

  1. -known Ag (ie, Toxoplasma gondii) on slide
    -incubate with patient serum 25 min.
    -wash unbound serum away
    -incubate with conjugated anti-Hu gamma globulin ~25 min.
    -wash unbound Ab away
    detect in fluorescent microscope
  2. -Antibody mixed into gel with specimens in well
    -screening for presence of antigen
    precipitate forms band around well which
    indicate presence of antigen
    -size of band relative to concentration of antigen
  3. -antigen coated to plastic well
    -protein blocks remaining plastic surface
    -Serum added(primary antibody)
    -if antibodies(bind antigen)
    -if no antibodies(antigen not bound)
    -Indicator antibody(enzyme-linked anti-Ig antibody binds primary antibody)
    -Substrate(specific for enzyme linked to secondary antibody,enzyme causes substrate to change color)
    -Reactions(color change=
    antibody in serum,no color change=
    no antibody in serum)
  4. -results in mix soluble antigen and antibody
    -excess antigen or antibody--no precipitate
    -zone of equivalence--precipitate forms
  5. -Bind to cell
    -Visualize under UV light

5 True/False questions

  1. Test issues (Sensitivity)eliminate false positives


  2. RPR - syphilis test (indirect, screening) many false positives-Ag is cardiolipin (beef liver)
    -specific test uses Ag from T. pallidum


  3. Hemagglutination-Known antigen in fluid with Unknown specimen added
    -Agglutination =positive reaction
    -No agglutination=negative reaction
    -Similar to qualitative
    -Diluted samples of antibody
    -Measure amount of agglutination for each dilution


  4. Agglutination reactions-using cells with antigen on them
    -naturally particulate antigens


  5. Direct Ag detection of Fluorescence microscopy procedure-patient sample on slide
    -ethanol to fix and kill
    -incubate with conjugated Ab ~25 min.
    -wash unbound Ab away
    -detect in fluorescent microscope


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