Chapter 5 Methods and Strategies

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Created by:

mcvaysm  on October 11, 2011

Subjects:

neuroscience

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Chapter 5 Methods and Strategies

Brain function vs behavior
circuits within the brain perform functions, not behaviors
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Brain function vs behavior circuits within the brain perform functions, not behaviors
excitotoxic lesion a brain lesion produced by intracerebral ijection of an excitatory amino acid, such as kainic acid
sham lesion a placebo procedure that duplicates all the steps of producing a brain legion except for the one that actually causes brain damage
fixative a chemical such as formalin; used to prepare a and preserve body tissue
formalin the aqueous solution of formaldehde gas; most commonly used fixative
microtome an instrument that produces very thin slices of body tissue
MRI magnetic resonance imaging
fMRI functional MRI, measures signal changes in the brain due to chanign neural activity
DTI diffusion tensor imaging- white matter connectivity of the brain
CT computed tomography
PET positron emission tomogrpahy
SPECT single photo emission computed tomogrpahy
-type of nuclear imaging test that shows how blood flows to tissues and organs
EEG electroencephaogram
magnetoencephalography procedure that detects groups of synchrounsuly activated neurons by means of the magnetic field induced by their electrical activity.
uses SQUIDS
FOS a protien produced in the nucleus of a neuron in response to synaptic stimulation
Transcranial magnetic stimulation (TMS) stimulation of the cerebral cortex by means of magnetic fields produced by passing pulses or electricity through a coil of wire placed next to the skull; interferes with the functions of the brain region that is stimulted
Recording metabolic and synaptic activity Functional imaging- a computerized method of detecting metabolic or chemical changes in particular regions o the brain
PET - use a device that reveals the locailzation of a radioactive tracer in a living brain
Targeted Mutations recently developed
-mutated genes produced in the laboratory and inserted into the chromosomes of mice (knockout genes)
-these mutate genes are defective: they fail to produce a functional protien
antisense oligonucleotides a modified strand of RNA or DNA that binds with a specific molecule of messenger RNA and prevents it from producing its particular protein

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