| Term | Definition |
|---|
| renaturation kinetics differential equation | dC/dt = -kC2 |
| renaturation kinetics equation | C/Co = 1/(1 + kCot) |
| renaturation kinetics plot | fraction reassociated [1 - (C/C0)] vs initial concentration x time [Co*t] |
| Southern blotting | number of genomic copies |
| in situ hybridisation to polytene chromosomes | genomic location of specific DNA |
| moderately repetitive genome fragment | some genes and transposable elements |
| average eukaryotic chromosome size | 50 MBp |
| yeast genome size | 13MB |
| # yeast genes | ~6000 |
| yeast gene density | 1 gene per 2kb |
| yeast genome ORF percentage | 70% |
| RNA seq | make cDNA with dT and random primed, add adaptors, Illumina sequencing |
| Illumina sequencing stats | 25-36bp; 5million reads/lane; 40 million reads/slide; 1GB sequence |
| Illumina sequencing method | add adaptors, attach DNA to slide, make DNA bridge, make double stranded, denature, amplify, first base synthesis, image, repeat |
| % non-repetitive yeast genome transcribed | 74.5% |
| C. elegans genome size | 97 MB |
| # C. elegans genes | >19,000 |
| C. elegans orthology with other organisms, yeast | 40, 24% |
| C. elegans gene density | 1 gene per 5kb |
| C. elegans average intron count | 5 |
| C. elegans exon percentage | 27% |
| Drosophila band number | ~5000 |
| # Drosophila genes | 13,700 |
| Drosophila gene density | 1 gene per 9kb |
| Arabidopsis genome size | 125 MB |
| # Arabidopsis genes | 25,500 |
| Arabidopsis gene density | 1 to 38 genes per 100kb |
| Arabidopsis gene orthology | 70% |
| Human genome size | 3 GB |
| # Human genes | 20-25,000 |
| Human gene density | 9 genes per MB |
| Human genome composition | Exons, 1%; Introns 24% |
| Mouse genome size | 2.5 GB |
| Mouse# mouse genes | 20-25,000 |
| Human mouse synteny | 90% |
| Cosmid size | 40 KB |
| BAC size | 200 KB |
| YAC size | 500 KB |
| # yeast introns | ~300 |
| Drosophila average intron count | 4 per gene |
| Arabidopsis average intron count | 4 per gene |
| Human average intron count | 6 per gene |
| Human coding content | ~1.5% |
| Drosophila coding content | ~24% |
| Arabidopsis coding content | ~29% |
| Mouse average intron count | 6 per gene |
| mouse-human neutral subst rate | 1/2 nucleotide per site |
| histones | H1, H3, H2B, H2A, H4 |
| Histone: DNA mass ratio | 1:1 |
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