5 Written Questions

1. EMB agar - selective for lactose fermentation
   Simmons Citrate agar - selective for citrate utilization
   Mannitol salt agar - selects for gram positive cocci
2. Tryptic soy Agar
   Neutral or N/A
   Used for routine cultivation of many types of bacteria.
   pH is neutral 7%.
3. E. coli...
4. Sabouraud Agar
   Selective
   Slightly acidic pH (5.6)
   General purpose media for the routine cultivation of fungi.
5. Identification of Pseudomonas
   Differential -
   Used to distinguish Pseudomonas aeruginosa on the basis of the pigment production
   Positive results = blue/green pigment

5 Matching Questions

1. Catalase test *
2. TSB
3. BAP
4. OF Basal media with glucose *
5. Name the 5 n/a media

1. a Tryptic Soy Broth
   N/A media
   Neutral 7
   Cultivation of many types of bacteria
2. b Oxidation - Fermentation Test
   Differential
   pH indicator=Bromthymol blue
   Oxidative = yellow in open tube
   --> green in the unopen tube
   Fermentative = yellow in both tubes
3. c N/A
   test for the production of catalase
   Positive result = Oxistrip turns blue/black within 20 seconds
4. d Hemolysins test
   Blood Agar Plate
   Differential
   TSA with 5% sheeps blood (used to test for the production of hemolysins.)
   Reaction = clearing of rbc's
5. e TSA
   TSB
   Oxidase Test
   Catalase Test
   Coagulase Test

5 Multiple Choice Questions

1. BAP
   Thioglycollate Broth
   Motility Media
   Fermentation Broth
   Pseudomonas F and P
   OF Basal media w/glucose
   Phenylalanine agar
   TSI agar
   Decarboxylation broth
   Starch agar
   Skim milk agar
   DNAse test agar
   Tryptone broth
   MR/VP broth
   1. Name the 3 Diff. and Sel. Media
   2. Name the 5 n/a media
   3. Name the 15 Differential Media
   4. Name the two selective media
2. Starch Hydrolysis Test
   Differential
   Flood media with Iodine after incubation
   Positive result - clearing around growth zone of hydrolysis
   1. 7% NaCL Agar
   2. EMB Agar
   3. Starch agar
   4. TSI agar
3. Reducing Media
   Differential
   For anaerobic bacteria
   used to differentiate oxygen requirements
   1. Thioglycollate Broth
   2. Tryptone broth
   3. Decarboxylation broth
   4. MR/VP broth
4. MR-VP test (Methyl Red/Voges-Proskauer test)
   Differential -
   pH indicator Methyl red
   add after incubation VP reagent A and VP reagent B
   Positive MR (Methyl red test) = stays red
   Positive VP (Voges-Proskauer test) = pink or red
   1. BAP
   2. Tryptone broth
   3. EMB Agar
   4. MR/VP broth
5. Staphylococcus aureus...
   1. Name a + for Acid-Fast
   2. Name a Gram - bacillus
   3. Name a Gram + bacillus and + for endospores
   4. Name a Gram + coccus and + capsules

5 True/False Questions

1. TSI agar → Production of Hydrogen Sulfide
   Triple sugar Iron agar
   Differential
   pH indicator phenol red
   Positive for the production of hydrogen sulfide - turns black

   True        False

2. Tryptone broth → MR-VP test (Methyl Red/Voges-Proskauer test)
   Differential -
   pH indicator Methyl red
   add after incubation VP reagent A and VP reagent B
   Positive MR (Methyl red test) = stays red
   Positive VP (Voges-Proskauer test) = pink or red

   True        False

3. Name a Gram + bacillus and + for endospores → Staphylococcus aureus...

   True        False

4. Skim milk agar → Casein Hydrolysis test
   Differential
   Positive results= clearing around growth zone of hydrolysis

   True        False

5. Name the two selective media → BAP
   Thioglycollate Broth
   Motility Media
   Fermentation Broth
   Pseudomonas F and P
   OF Basal media w/glucose
   Phenylalanine agar
   TSI agar
   Decarboxylation broth
   Starch agar
   Skim milk agar
   DNAse test agar
   Tryptone broth
   MR/VP broth

   True        False