| Term | Definition |
| biotechnology | the use of modern devices to study living things, especially DNA |
| restriction enzyme | special protein that can cut DNA into pieces |
| restriction fragment | pieces of DNA left after a restriction digest |
| restriction site | palindromic location on DNA where a restriction enzyme can cut the DNA |
| gel electrophoresis | process that separates DNA based on size and electrical charge |
| buffer | salty liquid which is used in gel electrophoresis |
| agarose | protein used to make gel which separates DNA fragments |
| plasmid | small circular piece of DNA found in bacteria |
| transformation | process where foreign DNA is added to a new organism |
| recombinant DNA | DNA formed by joining DNA from 2 different sources |
| DNA ligase | enzyme that will bond the backbones of two peices of DNA together |
| palindrome | section of DNA that reads the same forward and backward |
| DNA fingerprint | banding pattern produced from gel electrophoresis of restriction fragments |
| PCR | process for making many copies of DNA in a test tube |
| Sanger method | way to figure out the order of the nitrogen bases on a piece of DNA |
| recombinant plasmids | circular peices of DNA joined from two different sources |
| polymerase | enzyme that helps to copy DNA |
| thermocycler | machine that performs PCR |
| Human Genome Project | project to determine the order of the nitrogen bases on a strand of DNA |
| Kary Mullis | inventor of PCR |