Microbiology I - STUDY GUIDE EXAM 1

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LBosher  on November 22, 2011

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DIFS SEMESTER FOUR

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Microbiology I - STUDY GUIDE EXAM 1

Bacillus
Rod shaped bacterium
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Terms

Definitions

Bacillus Rod shaped bacterium
Bacteriology study of microorganisms called bacteria
Biology study of all living organisms both plant and animal
Botany study of plant live
Capsule A slimy mucilaginous (mucous-gelatin like) coating outside the cell wall
Cell membrane A semi-permeable membrane found just inside the cell wall. Serves to control the osmosis of substances into and out of the cell.
Cell Wall It is responsible for maintaining the size & shape of a living cell. Also serves as a reference point for describing the location of other cell parts.
Coccus Spherical or round bacterium
Conjugation A form of sexual reproduction utilized by bacteria
Cytoplasm The semi-fluid material inside the cell
Diplobacillus Two rod shaped cells forming a pair
Diplococci Two Coccus shaped cells clinging together
Endospore serves as a source of protection when environmental conditions are not favorable for survival or growth. Such as in conditions of heat drying, poor nutrient supply and chemical exposure.
Eucaryotic microorganisms with a well-defined or "true" nucleus with a nuclear membrane.
Fimbriae (Also known as Pili) Found externally but extend through the cell wall to attach to the cell membrane. Serves as a mechanism of attachment of the cell to a surface. Serves to mediate a form of sexual reproduction utilized by bacteria.
Flagella Found external of the cell wall they provide motility for the cell
Germination When the conditions for growth improve the Endospore will then convert into a living, growing, vegetative cell.
Granules Inclusion bodies; Serve as a storage place for food reserves or as an accumulation point for substances formed in excess of the immediate needs of the cell.
Immunity The condition in which protection either natural or acquired, is obtained against disease causing agents.
Immunology The study of immunity
Inclusion bodies Granules; Serve as a storage place for food reserves or as an accumulation point for substances formed in excess of the immediate needs of the cell.
Mesosome Aids the cell in reproduction by separating the new copies o DNA. It serves as the location of enzymes necessary for bacterial reproduction.
Microbiology The study of microorganisms and their effects on other living organisms.
Micro meter the most used unit of measurement for bacteria. It is 1 millionth of a meter or 1/1000 of a millimeter. Also 1/25,400 of an inch.
Microorganism Any plant or animal that must be studied using a microscope.
Mold multi-cellular Eucaryotic organisms. Reproduce by conidia or zygote formation
Morphology The size & shape and arrangement of cells.
Mycology The study of fungi. (mold yeast)
Nuclear body the location of the bacterial DNA. It functions as the brain of a cell it a carries the inheritable characteristics of the cell, it controls metabolism.
Pili (Also called Fimbriae) - Found externally but extend through the cell wall to attach to the cell membrane. Serves as a mechanism of attachment of the cell to a surface. Serves to mediate a form of sexual reproduction utilized by bacteria.
Procaryotic Do not have an organized nuclear body within a membrane.
Protozoology The study of single cell animals.
Ribosomes The site of protein synthesis. It builds the protein that are necessary for cell growth and metabolism.
Rickettsiology The study of Rickettsia - small obligate intracellular parasites.
Sarcinae A cubical packet of eight spherical cells.
Spirillum A spiral shape bacterial that remains ridged when in motion.
Spirochete A spiral shape bacteria that bends when in motion.
Sporulation when a cell is stimulated to form an Endospore.
Staphylococci Three or more cocci forming a cluster
Streptobacillus Three or more bacilli attached end to end to form a chain
Tetrad A grouping of four shaped Coccus shaped cells
Vibrio A curved or comma shape.
Virology The study of viruses
Yeast A fungi. A single-celled eucaryoic organism; reproduces by budding.
Zoology The study of animal life.
Explain why it is important for an embalmer to study pathology. (Microbiology)- To recognize the importance of bacterial as related to human digestion & nutrition.
- To understand how microbes function as normal flora to protect humans against infection by more virulent organisms.
- To recognize the benefits of bacteria such as breakdown of human sewage
- To understand the importance of bacteria in the process of decomposition.
- To understand how the transmission of diseases so to protect against the transmission of diseases in the prep-room.
- Recognize the microbiological conditions that may require special procedures.
- Demonstrate knowledge of the terminology to enable competent communications.
- Understand why cooperation in the community to control disease is important.
Describe bacteria as we did under bacteriology on page 2 of the outline. A group of organisms that:
- Are singled celled
- They lack a nuclear membrane
- They do not contain chlorophyll
- May be able to move about independently
- Reproduce by an asexual process called binary fission.
What other organisms besides bacteria are prokaryotic None. Only blue-green algae
How do yeasts reproduce? By budding
How do molds reproduce? By conidia or zygote formation (spores)
Describe rickettsia and viruses in detail.Rickettsia
- Small obligate intracellular parasites
- Cause disease characterized by fever and skin rash
- Transmitted to humans by lice, fleas, mites and/or ticks
Viruses
- Contain only one type of nucleic acid, either RNA or DNA Non-cellular; therefore they are obligate intracellular parasites
- Require an electron microscope to be seen
- Depend on host survival in order to survive
What is the lowest division of the animal kingdom? Single cell animals
What causes malaria and amoebic dysentery? Single cell animal / parasites
What reproduces by binary fission? Bacteria
What two groups lack a nuclear membrane? Bacteria and Blue-Green Algae
What two groups are obligate intracellular parasites? Rickettsia and Viruses
Where would only one type of nucleic acid be found? In a virus
Where would a protein coat be found? On a virus
What requires an electron microscope to be seen? Virus
What science would include a study of antigen-antibody reactions? Immunology
Thoroughly describe binomial nomenclature.- a system developed by botanist Carolus Linnaeus
- It provides a first name call
- the genus is always capitalized and the species is not.
-The writing or typing the name, the entire name is either italics or underlined.
- It can be seen in "Bergey's Manual of Systematic Bacteriology" in the following order: Kingdom, phylam (division), class, order, family, genus, speciesed a "genus" and a second name call a "species". When writing the name
What is the first name of a bacterium? Genus
What is the second name of a bacterium? Species
Give the correct Sequence of the naming system used for bacterium. Kingdom, Phylam, Class, Order, Family, Genus, Species.
How many Micrometers are in a millimeter? 1000
Compare a µM (micro-meter) to an inch. 1 micro-meter is equal to 1/25
400 of an inch
List the groups of microorganisms from largest to smallest. - Protozoa - 100 µM
- Yeast & molds (fungi) 40 µM
- Bacteria - 1 -20 µM
- Rickettsia & mycoplasmas - 0.4 µM
- Virus - 0.01 µM
Which shape of bacteria shows the most diversity in cellular arrangement? Cocci
Which shape of bacteria shoes the least diversity in cellular arrangement? Spirals
What maintains the size and shape of a bacterial cell? Cell Wall
What part of a bacterial cell serves as a reference point for describing the location of other cell parts? Cell Wall
Where is the cell membrane found? Just inside the cell wall
What controls the osmotic movement of materials into and out of a bacterial cell? Cell Membrane
What provides the secretory and ecretory functions for a bacterial cell? Cell Membrane
Describe the capsule. A slimy mucilaginous (mucous- gelatin like) coating outside of the cell walls.
List two ways a capsule protects a bacterial cell. - By acting as a buffer against harmful chemical
- By resisting dehydration
List two ways a capsule increases the virulence of a bacterium. - By inhibiting or retarding phagocytosis
- Helps the cell to attach to surfaces
What are the two functions of the Mesosome? - It aids the cell in reproduction by separating the new copies of DNA
- It serves as the location of enzymes necessary for bacterial reproduction
What acts the brain of a bacterial cell? Nuclear body
What controls bacterial metabolism? Nuclear body
Where is the bacterial DNA located? Nuclear body
What does bacterial DNA do? - It carries the inheritable characteristics of the cell
- It controls metabolism
Why do some bacteria form spores? Serves as a source of protection when environmental conditions are not favorable for survival or growth.
What genera of bacteria produce spores? Bascillus & Clostridium
List the diseases that are caused by the spore-forming bacteria. - Gas gangrene
- Tissue gas (in the dead)
- Anthrax
- Botulism
- Tetanus
What serves as the storage place for food reserves and other excess materials in the bacterial cell? "Inclusion bodies" also called "granules"
What part of a bacterium enhances its ability to colonize and infect? "Pili" also called "Fimbriae"
What two structures of a bacterial cell make it better able to attach to surfaces? Capsule andPili

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