| Term | Definition |
|---|
| Oxidative Phosphorylation | ATP formation by discharge of membrane potential |
| PMF | Proton Motor Force (membrane potential, cell membrane impervious to ion diffusion) |
| Functions of ETC | ATP generation by OP, Re-oxidation of NADH + H+ and FADH2 |
| Electron Transport Chain | generates energy through PMF for ATP by OP |
| H+ translocation? | Creates [H+] gradient |
| Terminal Electron Acceptor? | O2 oxidizes Cyt a |
| Why PMF for OP? | Flow of ions across membrane used to drive ATP synthesis |
| PMF for Rotary Catalysis? | [H+] through ATPase uses energy of PMF |
| ATPase? | Sole H+ channel, chemiosmosis |
| PFK regulation of ATP synthesis? | Speeds up or slows down |
| Allosteric regulation of PFK? | ADP +, ATP -, Citric Acid -: all change tertiary structure |
| ETC uncoupler (2,4-DNP) | Disconnect ETC from ATP synthase |
| 2,4-DNP membrane potential | decreases periplasmic [H+] |
| 2,4-DNP pH gradient | same |
| 2,4-DNP O2 consumption | increases |
| 2,4-DNP ATP synthesis | decreases |
| 2,4-DNP PFK activity | increases |
| 2,4-DNP Citric Acid Cycle | increases |
| ETC inhibitor (Azide) | Blocks flow of electrons in ETC |
| Azide membrane potential | slowly decreases |
| Azide pH gradient | decreases |
| Azide O2 consumption | decreases |
| Azide ATP synthesis | slows |
| Azide PFK activity | 1st increases (ADP), then obligate aerobe dies, facultative anaerobe switches to fermentation (levels off) |
| Azide Citric Acid Cycle | 1st increases (PFK), then obligate aerobe dies, facultative anaerobe switches to fermentation (shuts down) |
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