5 Written questions
5 Matching questions
- What does transferase do?
- What is the limit dextran?
- What is glycogen?
- How is UDP-glucose formed?
- What is the overall reaction of UDP glucose formation?
- a A very large, branched polymer of glucose residues. Most of the glucose residues are linked by alpha 1,4 glycosidic bonds. branches about every 10th residue are linked by alpha 1,6 glycosidic bonds
- b Glucose-1-phosphate +UTP =>UDP-glucose + PPi
- c The 4th glucose residue from the branch glucose
- d UDP-glucose is synthesized from Uridine triphosphate and glucose-1-phosphate via the enzyme UDP-glucose pyrophosphorylase
- e Transferase transfers the 3 terminal glucose residues from outer branch to the center, main linear branch
5 Multiple choice questions
- PPi+ H2O=> 2Pi
- Increases the number of terminal glucose residues which are site of action for glycogen phosphorylase and synthase. Branching increases rate of glycogen synthesis and degradation
- Uridine diphosphate is a nucleotide composed of uracil, ribose and pyrophosphate
- Glycogenin is both an enzyme and its substrate consisting of two 37 kD subunits. Each subunit catalyzes addition of a glycosyl unit from UDP glucose to Tyr 194 of other subunit. Subsequently, 7 more glycosyl units are added so that 8 glycosyl units are attached to Tyr 194 for each subunit
- Must be at least 4 residues away from other branches. Links are alpha 1,6 glycosidic bond
5 True/False questions
What are the properties of branching? → A glycosyl unit from UDP glucose is transferred to the hydroxyl group at the C4 of the terminal glucose residue forming a alpha 1,4 glycosidic bond. Requires a glycogen molecule that is a minimum of 4 residues to which a glycosyl will be added
What enzymes are involved in Glycogen degradation? → Glycogen phosphorylase, Transferase, alpha 1,6 glucosidase, phosphoglucomutase
What are the properties of Glycogen synthase? → A glycosyl unit from UDP glucose is transferred to the hydroxyl group at the C4 of the terminal glucose residue forming a alpha 1,4 glycosidic bond. Requires a glycogen molecule that is a minimum of 4 residues to which a glycosyl will be added
What does alpha 1,6 glucosidase do? → Phosphoglucomutase is a phospho enzyme and converts Glucose 1 phosphate to glucose 6 phosphate for further metabolism
What converts Glucose 6 phosphate into glucose in liver? → Alpha 1,6 glucosiase hydrolyzes the alpha 1,6 glucosidic bond to release a free glucose molecule