5 Written Questions

1. locus with two or more alleles that are present in more than 1% of a species members
2. rudimentary DNA packaging unit
3. all the omponents needed for an automated acquisition of a set of data
4. a. bind to enhancer DNA in a sequence specific way
   b after binding, be able to itneract with other proteins to activate transcription
5. purified fragments of single-stranded DNA 25 to several thousand nucleotides in length that are subsequently labeled with a radioactive isotope or fluorescent dye

5 Matching Questions

1. serial analysis of gene expression
2. insertional inactivation
3. minimal tiling path
4. induction
5. hierarchical shotgun sequencing

1. a SAGE
   synthesize small cDNA tags about 15 bp in length from 3' ends of mRNA and link these tags together in 1000 bp DNA that can then be sequenced
2. b process by which a speciic molecule stimulate synthesis of a given protein
3. c 1. construct library by fragmenting target genome and cloning it into large fragment cloning vector
   2. organize genomic DNA fragments represnted into physical map and select and sequence BAC clones on a minimum tiling path by random shotgun strategy
   requires front end work to create physical map of BAC clones
4. d minimally overlapping set of BACs
5. e process of engineering an insert to interrupt a host gene

5 Multiple Choice Questions

1. recomb DNA molecules are added to suspension of prepared E.coli under conditions that favor entry
   1. how do host cells take up DNA
   2. what is order of lac operon?
   3. what does sigma subunit do?
   4. RNA polymerase core units
2. show location of genes, genetic markers, centromers, telomerse and other points of interest along chromosome of organism
   1. sequence maps
   2. idiograms
   3. chromosomal maps
   4. nucleosome
3. it decreases the activity of adenyl cyclase, which is what converts ATP into cAMP
   so when glucose is present cAMP is low and when glucose is absent cAMP is high
   catabolite repression
   1. how do host cells take up DNA
   2. how to make restriction map
   3. how to genotype SSR
   4. how does glucose affect cAMP?
4. from N terminus to C terminus
   1. in what direction does mRNA translation go?
   2. where does methylation of DNA occur
   3. explain replicating mRNA to cDNA
   4. what is order of lac operon?
5. up to reproductive clone
   take embryos and let it grow into blastocyst
   remove the ES cells and place in culture and let differentiation go on
   1. ethidium bromide
   2. genetic varients
   3. therapeutic cloning
   4. molecular cloning

5 True/False Questions

1. Rho dependent termination → protein factor Rho recognizes sequence in newly transcribed mRNA and terminates transcription by binding to RNA and pulling it away from RNA polymerase enzyme

   True        False

2. ribonucleotide complexes → miRISCs
   have diverse functions depending on ARgonaute protein they possess and extent of sequence complementarity between guide miRNA and target
   perfect complementarity cause mRNA cleavage
   less complementariy is usually inhibition o ftranslation
   cotranslation protein degradation
   inhibition of translation elongation
   premature termination of translation
   inhibiton of ranslation initiation

   True        False

3. activators → chromosome-like carriers that ensure transport, replication and purificatin of individual inserts

   True        False

4. rho independent termination → sequence of about 20 bases in RNA with a run of 6 or more Us at end forms a stem loop that serves as signal for release of RNA polymerase from completed RNA

   True        False

5. DNA sequencing → group of replicated DNA molecules cause all copies are identical

   True        False