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DNA ligase

Which of the following enzymes can create a bond between adjacent, unjoined nucleotides?

small circlets of DNA found in bacteria

Frequently, genetic engineers use plasmids, which are _____.

Gene cloning occurs when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid.

What is gene cloning?

provide a site for complementary base pairing so that pieces of DNA can be linked together

"Sticky ends" are very useful in genetic engineering because they _____.

a restriction enzyme

An enzyme that "cuts" DNA at a specific sequence of nucleotide bases is called _____.

destroy foreign DNA

The "normal" function of restriction enzymes is to _____.

a restriction enzyme and a ligase

The recombinant molecule below was generated using which two enzymes?

insert a foreign piece of DNA cut with the same restriction enzyme

Shown below is the effect of a restriction enzyme known as EcoRI. Cutting with this enzyme allows scientists to _____.

the generation of short fragments of a single-stranded DNA left at the end of a DNA molecule

What does the term sticky end refer to in genetic engineering?

the fragments will bond to other fragments with complementary single-stranded ends

When a typical restriction enzyme cuts a DNA molecule, the cuts are staggered so that the DNA fragments have single-stranded ends. This is important in recombinant DNA technology because _____.

unpaired bases produced by a trstriction enzyme

The so-called sticky ends of a plasmid or bacterial chromosome are _____.

restriction enzymes

DNA used in recombinant DNA techniques is first cut into fragments by _____.

a plasmid or other agent used to transfer DNA into a living cell

What does the term vector refer to in genetic engineering?

genomic library

Copies of cloned genes are stored in a _____.

work backword from mRNA to make a version of the gene without introns

Bacteria lack RNA-splicing machinery, which means they are unable to splice out introns from eukaryotic genes. To engineer a bacterium to produce a eukaryotic protein, it is necessary to synthesize a gene without introns. If you know the nucleotide sequence, you can _____.

cDNA

DNA synthesized using an RNA template is called _____.

reverse transcriptase

Which enzyme makes DNA from an RNA template?

it helps identify genes that have been inserted into bacterial plasmids

What purpose does a nucleic acid probe serve?

they both undergo complementary base pairing

In which of the following ways are sticky ends and nucleic acid probes alike?

pharming

The production of genetically identical animals that are carrying recombinant human genes for pharmaceutical purposes is called _____.

the mRNA was not spliced as it is in eukaryotes

A eukaryotic gene was inserted into the DNA of a bacterium. The bacterium then transcribed this gene into mRNA and translated the mRNA into protein. The protein produced was useless; it contained many more amino acids than the protein made by the eukaryote. Why?

use bacteria as "factories" for protein products

Cloning human genes into the plasmids of bacteria has enabled scientists to _____.

A harmless variant, a natural mutant, was used to stimulate an immune response

How was the smallpox vaccine produced?

A rat with rabbit hemoglobin genes

Which of the following would be considered a transgenic organism?

the inserted ("foreign") gene is expressed in the host organism

Transgenic organisms are only scientifically or commercially useful if _____.

the conditions would be unfavorable to their survival

Genetically modified organisms include microbes that possess enzymes promoting antibiotic resistance. This is a problem that concerns many individuals, with the rise of antibiotic-resistant organisms. However, these organisms do not pose a risk to public health. Why?

it adds a functioning version of the defective gene to the cells of an individual

What does the process of gene therapy involve?

isolating and cloning the normal gene

To date, the "easy" part of gene therapy has been _____.

compares the DNA banding patterns of small segments of the genome

DNA profiling is a forensic procedure being utilized to identify individuals. This technique _____.

particular nucleotide sequence whose inheritance can be followed

A genetic marker is a _____.

it can make a large amount of DNAA from a tiny amount

What is the function of the polymerase chain reaction (PCR)?

it allows for the separation of fragments of DNA

What is the purpose of gel electrophoresis?

short tandem repeat

DNA profiles utilize _____ DNA sequences

use the polymearse chain reaction

Archaeologists unearthed a human skull with a small dried fragment of the scalp still attached. They extracted a tiny amount of DNA from the scalp tissue. To obtain sufficient DNA for an analysis of the ancient human's genes, they could _____.

the baby's...Sam's and Becky's

The patterns of bands on DNA profiles are sometimes called genetic fingerprints. Imagine that DNA profiles are used to determine whether Sam could be the father of Becky's baby. Sam is the father if _____ genetic fingerprint shows bands present in _____ genetic fingerprint.

the presence of various-sized fragments of DNA

DNA profiles used as evidence in a murder trial look similar to supermarket bar codes. The pattern of bars in a DNA profile shows _____.

96%

Comparison of whole genome sequences shows that we share _____ of our genome sequence with our closest relative.

rice

The current estimate for the number of genes in the human genome is about 20,500. Which of the following organisms has almost twice as many genes as humans?

transposable elements can move around in DNA

Barbara McClintock, a 1983 Nobel Laureate in Physiology or Medicine, found a particular nucleotide sequence that repeated itself on different chromosomes in different mouse skin cells. This supported the idea that _____.

We have a lot of "junk" DNA that does not code for anything

If the human genome is so large, relative to the size of other organisms' genomes, why do we have so few genes?

shotgun cloning

Celera Genomics implemented a faster way of sequencing the human genome. What was this method?

the genetic and physical mapping stages can be skipped

The shotgun cloning technique differs from the original method of sequencing the human genome because _____.

Isolate any food or drink that seems to influence the expression of the gene of interest

A particular digestive disorder is noted in members of a family. How would you approach the study of this disorder from a strictly proteomic angle?

Neanderthals may have had the ability to speak

Analysis of human and Neanderthal DNA have revealed that _____.

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