| Term | Definition |
|---|
| investigate the mechanism of UV inactivation, photorepair, dark repair of e.coli and Crypto parvum with the endonuclease sensitive site assay | objective of research |
| For photoreactivation, samples were irradiated by fluorescent lamps within 5 minutes after 99.9% inactivation by UV irradiation. For dark repair, sample kept in darkness for 25 hours after UV irradiation. For the animal infectivity assay of C. parvum, a low pressure UV lamp and fluorescent lmap were used for UV inactivation. the colony forming ability for E. coli was assessed using desoxychloate-acidm medium and the number of colony formed were counted. | how were the experiments designed |
| UV-B and UV-C radiation is effected through the formation of lesions in the genomic DNA of the organisms. Lesions would inhibit the normal replication of DNA. | how does UV inactivate organisms |
| photorepair: repair of pyrimidine dimers in the DNA; excision repair: also named dark repair because they can repair the damage of DNA without light | two main types of repair of UV-induced damage |
| Endonuclease sensitive site assay, it can determine UV-indcued pyrimidine dimers in the genomic DNA of microoganisms | what does ESS Assay measure and how it works |
| yes | was photo repair observed with e. coli and crypto |
| not observed with e coli, but was observed with C. parvum | was dark repair observed with e. coli and crypto |
| animal infectivity of C. parvum did not recover after either exposure to florescent light or storage in darkness | did repair allow the organisms to regain viability |
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