Why complete a blood film? (4)
blood mach down or concered about the accuracy of results; to classify anemia; platelet counts; cell morphology
When doing a blood film, what are some probs with slides? (4)
not mixing your blood tube well; not using a good technique; poor quality stain; platelet clumping
When making a blood film slide, what is important to start with?
a brand new slide
When doing a blood film slide, at what angle should you hold the extra slide?
What do you need to do to process an anemic blood sample?
increase the angle of the extra slide to 60*, to make the sample thicker
What do you need to do to process a dehydrated sample?
decrease the angle of the extra slide to 30*
What are the steps to processing a blood film?
put a drop of blood on one side of the slide; slide the extra slide to the drop of blood and let the drop of blood spread across the extra slide; at a 45* angle slide the extra slide towards the opposite end of the slide; allow blood to dry; stain your slide in Wrights stain; rinse; air dry; view
Describe the staining routine for processing a blood film slide.
swirl the slide into each stain color jar, blotting between each color; 5-5-5; then rinse
How is a differential count performed?
move the slide back and forth across the smear in a pattern that avoids covering the smae territory. identifyeach leukocyte that is encountered until 100 cells have been counted and sorted by type; record each cell type in the proper category on the counter
What is the relative differential count?
the percentage of each cell type that results, in a differential count
What layer, on the blood slide smear, is used for a differential count?
How is the absolute differential determined?
by multiplying the percentage of each cell type be the WBC to get the number of each type of leukocyte /ul
What do you do if nRBC are encountered while processing the absolute differential?
they should be recorded separately from the leukocytes and reported as the number of nRBC/100 WBC