| Term | Definition |
|---|
| UV spectrometry | Absorbance of energy due to electronic transitions in molecules |
| Chromophore | Part of molecule that absorbs UV or visible light |
| Common chromophores | Conjugated double bonds, aromatic rings |
| Uses | Quantitative analysis in combination with HPLC |
| 1st 4 physical components | Light source, slit, monochromator, slit |
| 2nd 3 physical components | Sample, light detector, computer |
| Measure of intensity of absorbance | Molar absorption coefficient |
| Auxochromes | Groups that modify absorption of neighbouring chromophores |
| Beer-Lambert law | Suggests a linear relationship between absorbance & concentration |
| Beer-Lambert equation | log10(Io/I)=A=ecl |
| Io/I | Intensity of incident light/intensity of transmitted light |
| ecl | molar absorption coefficient (L), concentration in moles, path length in cm |
Combine with other sets
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