What organisms grow on Chocolate agar? MacConkey? Mannitol Salt agar?
Chocolate: Has V factor (NAD) and X factor (hemin); fo Haem influenzuae and Neisseria gonorrhoeae
MacConkey: Gram neg rods
Mannitol Salt: S aureus, turns the surrounding yellow
During the lab demonstration, which organisms were able to grow in any conditions, aerobic, anaerobic, and increased CO2?
Anywhere: E coli
Anaerobic: B frag
Increased CO2: N gonorrhoeae
What are throat cultures looking to cultivate?
GAS, strep pyogenes
What are sputum specimens looking to cultivate?
Strep pneum, H flu, S aureus, enteric GNR for hospitalized pts
How do you know if a sputum sample is of good quality?
If there are nphils, then probably from the site of disease (nphils are pink on a Gram stain); if there are epithelial cells, then contamination from mouth, pharynx probably occurred