Biology Test 5(Part 1)

Set: Biology Test 5(Part 1)

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	Term	Definition
	Polymerase Chain Reaction	PCR stands for
	large	its a technique for creating ______ quantities of DNA
	small	how big is the amount of DNA needed to use PCR?
	95	the DNA sequence is heated to this temperature as the first step of PCR
	hydrogen	what bonds are broken in DNA?
	Primers	what is added to the reaction mixture in the second step of PCR?
	Taq DNA Polymerase	what enzyme is added along with lots of free nucleotides to extend the new complimentary strands from the Primers?
	thermas aquaticus	what bacteria species contains the TAQ?
	cheap	what makes PCR ideal in DNA profiling?
	Kary Mullis	who discovered PCR?
	Cetus Corp.	what corporation did Kery Mullis Work For?
	large pool of nucleotides	what is a requirement for PCR?
	makes DNA	what does Taq do?
	30	how many seconds does it take for hydrogen bonds to break in PCR?
	60	what temperature do the aimers attach to DNA strands in PCR
	20 seconds	how long does it take aimers to attach to DNA stands in PCR
	75	at what temperature does Taq replicate DNA best in PCR?
	2	cycles take this many minutes
	doubles	the DNA does this every cycle
	no	is Taq DNA Polymerase Denatured after cycles?