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5 Written questions

5 Matching questions

  1. What is the function of reverse transcriptase in retroviruses?
    A) It translates viral RNA into proteins.
    B) It uses viral RNA as a template for making complementary RNA strands.
    C) It uses viral RNA as a template for DNA synthesis.
    D) It hydrolyzes the host cell's DNA. E) It converts host cell RNA into viral DNA.
  2. Which of the following problems with animal cloning might result in premature death of the clones?
    A) use of pluripotent instead of totipotent stem cells
    B) abnormal regulation due to variant methylation
    C) use of nuclear DNA as well as mtDNA
    D) abnormal immune function due to bone marrow dysfunction
    E) the indefinite replication of totipotent stem cells
  3. A researcher needs to clone a sequence of part of a eukaryotic genome in order to express the sequence and to modify the polypeptide product. She would be able to satisfy these requirements by using which of the following vectors?
    A) a human chromosome
    B) a bacterial plasmid
    C) a YAC with appropriate cellular enzymes
    D) BAC to accommodate the size of the sequence
    E) a modified bacteriophage
  4. The fact that plants can be cloned from somatic cells demonstrates that
    A) differentiated cells retain all the genes of the zygote.
    B) differentiated cells contain masked mRNA.
    C) differentiation does not occur in plants.
    D) genes are lost during differentiation.
    E) the differentiated state is normally very unstable.
  5. In prophase I of meiosis in female Drosophila, studies have shown that there is phosphorylation of an amino acid in the tails of histones of gametes. A mutation in flies that interferes with this process results in sterility. Which of the following is the most likely hypothesis?
    A) Histone tail phosphorylation prohibits chromosome condensation.
    B) Histone tails must be removed from the rest of the histones.
    C) All proteins in the cell must be phosphorylated.
    D) Any mutation during oogenesis results in sterility.
    E) These oocytes have no histones.
  1. a C) it uses viral RNA as a template for DNA synthesis
  2. b B) abnormal regulation due to variant methylation
  3. c A) Histone tail phosphorylation prohibits chromosome condensation
  4. d C) a YAC with appropriate cellular enzymes
  5. e A) differentiated cells retain all the genes of the zygote

5 Multiple choice questions

  1. B) continuous transcripton of the structural gene controlled by that regulator
  2. E) The drug inhibits the specific thymine kinase receptor.
  3. A) T4 protein and T4 DNA
  4. D) repressor
  5. D) become capable of human-to-human transmission

5 True/False questions

  1. DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. What is the purpose of transferring the DNA from a gel to a nitrocellulose paper?
    A) to attach the DNA fragments to a permanent substrate
    B) to prepare the DNA for digestion with restriction enzymes
    C) to separate the two complementary DNA strands
    D) to separate out the PCRs
    E) to transfer only the DNA that is of interest
    D) no AUG in any frame

          

  2. Viral envelopes can best be analyzed with which of the following techniques?
    A) transmission electron microscopy
    B) staining and visualization with the light microscope
    C) immunofluorescent tagging of capsid proteins
    D) antibodies against specific proteins not found in the host membranes
    E) use of plaque assays for quantitative measurement of viral titer
    B) RNA polymerase must bind to the promoter, and the repressor must be inactive

          

  3. In animals, embryonic stem cells differ from adult stem cells in that A) embryonic stem cells are localized to specific sites within the embryo, whereas adult stem
    cells are spread throughout the body.
    B) embryonic stem cells have fewer genes than adult stem cells.
    C) embryonic stem cells are totipotent, and adult stem cells are pluripotent.
    D) embryonic stem cells are pluripotent, and adult stem cells are totipotent.
    E) embryonic stem cells have more genes than adult stem cells.
    B) The fragments must be separated by electrophoresis, the fragments must be treated with heat or chemicals to separate the strands of the double helix, and the probe must be hybridized with the fragment.

          

  4. Which of the following is most closely identical to the formation of twins?
    A) therapeutic cloning
    B) embryo transfer
    C) organismal cloning
    D) cell cloning
    E) use of adult stem cells
    C) organismal cloning

          

  5. Genetically engineered plants
    A) are banned throughout the world.
    B) are more difficult to engineer than animals.
    C) include a transgenic rice plant that can help prevent vitamin A deficiency.
    D) are being rapidly developed, but traditional plant breeding programs are still the only method used to develop new plants.
    E) are able to fix nitrogen themselves.
    C) include a transgenic rice plant that can help prevent vitamin A deficiency