Begin with the restriction digest of pARA and pKAN-R, briefly describe the steps required for construction your recombinant plasmid from pKAN-R and pARA restriction fragments. Explain your answer including the manipultions of these two plasmids annd their restriction fragments.
1. Use enzymes to cut plasmid
2. Insert the rfp gene from pKAN-R
3. Add bigger part from pARA to make pARA-R
4.Have it duplicate