Lab pratical Midterm Bacteria and Cultures

(PA) Pseudomonas Isolation Agar
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Terms in this set (22)
(PA) Pseudomonas Isolation Agar
Isolation of Psuedomonas/Quadrant Streak/Selective..... 1) growth on streaked line= Psudomonas + (2) No growth= Psuedomonas -
(BESC) BILE Esculin Agar
Isolation of Enterococcus/Fish Tail/Sel. and Diff.... (1) Black= enterococccus + (2) Not black = Entercoccus -
(BA) Blood Agar
Test for hemolytic activity/Quadrant Streak/Differential/ autoclaved,cooled,then blood added.... (1) No Growth Evident (2) clear zone around colony viewed from bottom= Beta hemolysis. (3) Green/Brown medium disscolorization viewed from top= Alpha hemolysis. (4) No color change in medium= Gamma hemolysis
(MAC) MaConkey Agar
Isolation of Gram - Enterics/Quadrant Streak/Selective and Differential.... (1) Purple= lactose fermenter (2) Colorless= non lactose fermenter (3) No Growth.
(GLU) Phenol Red Glucose Broth
Fermentative Ability/Dip and Sworl/ Biochemical Class/ pH > 7= red pH<7= yellow..... (1)RED=alkaline=K, yellow=acid=A (2) Gas bubble in Durham tube= +, no gas bubble = "-"
(MSA) Mannitol Salt Agar
Isolates Staphylococcus Species: identifies Staphylococcus aureus./Quadrant Streak/Selective and Differential. SA= 7.5% NaCl.. DA= MAnnitol ..pH= Phenol Red.... (1) Growth medium turns lemon-yellow= Staphylococcus aureus + (2) Growth medium is pink= Staphylococcus epidermidis + (3) No Growth= Staphylococcus -
Enterococcus faecalis
opputunist/in intestinal tract/ can cause bacteremia,cyctitis,VRE(Vancomycin Resistant Enterococci)
Pseudomonas aeruginosa
oppurtinisitc pathogen/fuity odor/ blue-green pigment/grows at 25* C.
Escherichia coli
vitamins K and B complex/ indicates fecal contamination/ 0157:H7= enterohemorrhagic
Proteus vulgaris
swarming colonies on plates/ oppurtunist in the urinary tract/ drug resistant/strains a problem/nosocomial infections/trimeyhopin and sulfa compunds.
Staphylococcus aureusdrug resitant. Methicillin resitant staphylococcus aureus/Vanocomycin resistant Staphylococcus aureasStaphylococcus epidermidisopputunist/found on skin/ produces a slime layer that enables them to become attached to hospital equipment.Clostridium sporogensanaerobic/intestinal water loss and hemorrhaging by destroying intestinal epithelial cells.Micrococcus lutuesappear in yellow cultureMicrococcus roseusappears in red cultureMicrococcusgram + cooci found in pairs of clusters,nonpathogenic,grows well at 25*CSerratia marcescenscoliform/soil and water/opportunist in AIDS/pneumonia alcohol/ at 25*C RED PIGMENTED.DNAaseBacillus megateriumlarge bacillus/cytological studies/produces casease/gives milk its white colorBacillus subtilisfound in soil/ bacitaracin and laundry detergent enzymes(protease)/paper production(amylease)/ resistant to chemicals and heat.(TSA) Trypticase Soy Agargrowth of wide range of bacteria,General/Varied innoculation/(TSB) Trypticase Soy Brothgrowth of wide range of bacteria/General/Dip and Swirl.(TGB) Thioglycolate Brothgrowth of anerobic microbes/BIOCHEMICAL/Dip and Swirl. (1)growth in top of aerobic layer=obligate aerobe (2) rowth throughout aerobic and anaerobic layers= faculative anaerobe (3) growth in bottom of anaerobic layer+ obligate anaerobe