The following series of questions are related to acetyl CoA, D-glyceraldehyde-3-phosphate, pyruvate, and lactate.
A) Draw the structures of these four compounds (the detailed structure of CoA is not needed, however the complete structure of phosphate needs to be presented) and link (order) them, relative to glucose (i.e., from glucose to compound 1, compound 2, and other compounds, but no other information is needed).
B) B) If C3 (#3 carbon) of the glucose molecule is labeled with 14C, circle the C on each these four compounds which are likely to be labeled. (Use the structures you draw for (A) above. No explanation is necessary.)
C) Describe the important features in each link in (A) above (total of four links, each
could be multiple enzymatic steps) among these four compounds and glucose in terms of formation, consumption and changes of coenzymes and ATP (no structures and enzymes are needed, but your answer needs to include the quantitative information, e.g., how many ATP consumed/generated per molecule of compound X)
The acid oxygen can be either protonated, i.e., with a H, or dissociated as shown. 2 pts for each correct structure
Methyl group can be presented as CH3-Detailed phosphate structure is needed Acetyl CoA without S is OK
C) Glucose to D-glyceraldehyde 3 phosphate: Two ATPs are consumed per glucose metabolized.
-D-Glyceraldehyde 3-phosphate to pyruvate: Two ATPs and one NADH are formed per D-glyceraldehyde 3-phosphate metabolized to pyruvate.
-Pyruvate to lactate: One NADH is consumed (re-oxidized) per molecule of pyruvate reduced to lactate.
-Puruvate to acetyl CoA: One NADH is formed and one CoA-SH (or HS-CoA) is consumed per molecule of pyruvate converted to acetyl CoA.