Upgrade to remove ads
Only $2.99/month

TCC Micro 150 Lab 25 & 34

Terms in this set (18)

nitrate respiration
the reduction of nitrate (NO3-) to nitrite (NO2-)
denitrification
the reduction of nitrite (NO2-) to nitrogen gas (N2) and nitrous oxide (N2O)
nitrate reductase
enzyme for nitrate respiration
durham tube
small tube inside test tube that catches nitrogen gas
nitrate broth
media used for Nitrate Respiration/Denitrification test
nitrate A and nitrate B; zinc powder and hydrochloric acid
reagents used for Nitrate Respiration/Denitrification test
positive results for nitrate respiration and negative for denitrification
-red color after first set of reagents
-bacteria posses nitrate reductase
positive results for nitrate respiration and denitrification
-no color after both sets of reagents
-gas bubble in durham tube
-produced nitrate reductase and nitrogen gas
negative results for nitrate respiration and denitrification
-red color after second set of reagents
nitrogen cycle
NO3- -----> NO2- ------->N2(g) +N2O
bacteria's ability to use nitrate as the terminal electron acceptor in low oxygen environments
purpose of nitrate respiration
enteric
lives in the intestines
pure culture
growth of a single kind of bacteria
coliform
gram negative, enteric, bacillus shaped bacteria that can indicate fecal contamination
enterobacteriaceae
large family of gram-negative, bacillus shaped bacteria that include normal and pathogenic enteric bacteria
purpose of using a miniature rapid biochemical system
save space and time in identifying unknown bacterium
enterotube II
miniature rapid biochemical system
enteric bacteria
what type of bacteria the system helps to identify
THIS SET IS OFTEN IN FOLDERS WITH...