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Microbiology Information to Know

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What MO is:
Catalase +
Modified Oxidase =
Furazolidone Disk S
Bactracin Disk R
Staphylococcus
What MO is:
Catalase +
Modified Oxidase +
Furazolidone Disk R
Bactracin Disk S
Micrococcus
What MO is
Catalase =
Modified Oxidase =
Furazolidone Disk S
Bactracin Disk R
Rothia (Stomatococcus)
What MO causes skin infections, food poisoning, scalded skin syndrome and toxic shock syndrome?
S. aureus
What staphylococcus causes UTI?
S. saprophyticus
What causes Aggressive endocarditis?
S. lugdunensis
What will identify S. aureus?
Coagulase +
Latex agglutination +
Grows on MSA: yellow colonies
What is associated with indwelling medical devices?
Cogaulase-Negative Staphylococci
What MO is
Coagulase =
Novobiocin R
S saprophyticus
What is sticky staph?
Rothia (stomatococcus)
What MO is:
Catalase +
Tube coagulase =
Ornithine decarboxylase +
PYR +
S. lugdunensis
What is the hemolysis for:
GAS
GBS
GPS C, F, G
GDS
Pneumo
Viridans
GAS and Gps C, F, G - Beta
GBS - Beta and gamma
GDS and Viridans - Alpha and gamma
Pneumo- Alpha
What MO is GAS and causes pharyngitis, scarlet fever, skin infections, rheumatic fever, glomerulonephritis, and streptococcal toxic shock syndrome?
S. pyogenes
What MO is GBS and causes neonatal sepsis and meningitis?
S. agalactiae
What causes pneumonia and meningitis?
S. pneumoniae
What causes endocarditis?
Viridans streptococci
What GDS that is bacteremia associated with colon cancer?
S. gallolyticus
S. bovis
What is:
A disk S
SXT R
PYR +
GAS (S. pyogenes)
What is:
Camp +
SXT R
Hippurate +
GBS (S. agalactiae)
What is:
Bile-Esculin +
Salt +
PYR +
Enterococci
What is:
Bile esculin +
Salt =
NonEnterococci Group D
What is
Optochin S
Bile soluble
S. pneumoniae
What is the hemlysis for:
Arcanobacterium
Bacillus anthracis
Bacillus Species
Corynebacterium
Listeria
Lactobacillus & Erysipelotrix
Beta
Gamma
Beta
Gamma
Beta
Gamma and Alpha
What is:
Gram Stain: chinese letters (pleomorphic rods)
Catalase +
Motility =
Corynebacterium
What is:
Motility + (25)
Catalase +
Bile esculin +
Umbrella pattern in semisolid media
tumbling in wet mounts
Esculin hydrolysis +
Hippurate/Camp +
Listeria monocytogeneus
What is:
Gram Stain: GP cocci
Hemylosis Beta or gamma
Camp/Hippurate+
GBS
What uses media
BAP, cystine-tellurite, Pai, Tinsdale and Loeffler.
Methylene bluestain: metachromatic granules in cells;
Elek test for toxin?
C. diphtheriae
What MO do you get when ingest contaminated food; cause variety of infections in neonates, pregnant women, and immunocompromised patients?
Listeria monocytogenes
What MO is:
Glucose +
Maltos =
Lactose =
Sucrose =
Nitrate =
DNAse =
Hydroxyprolylaminopeptidase +
gamma-Glutamyl-aminoeptidase =
Beta-Glactosidase =
Butyrate esterase =
N. gonorrhoeae
What MO is:
Glucose +
Maltos +
Lactose =
Sucrose =
Nitrate =
DNAse =
Hydroxyprolylaminopeptidase V
gamma-Glutamyl-aminoeptidase +
Beta-Glactosidase =
Butyrate esterase =
N. meningitidis
What MO is:
Glucose +
Maltos +
Lactose +
Sucrose =
Nitrate =
DNAse =
Hydroxyprolylaminopeptidase +
gamma-Glutamyl-aminoeptidase =
Beta-Glactosidase +
Butyrate esterase =
N. Lactamica
What MO is:
Glucose =
Maltos =
Lactose =
Sucrose =
Nitrate +
DNAse +
Hydroxyprolylaminopeptidase =
gamma-Glutamyl-aminoeptidase =
Beta-Glactosidase =
Butyrate esterase +
M. catarrhalis
What MO is:
X and V factor +
No hemolysis?
H. influenzae
What MO is:
V factor +
No hemolysis?
H. parainfluenzae
What MO is V and X factor and has hemolysis?
H. haemolyticus
What MO is V factor and has hemolysis?
H. parahaemolyticus
Which MO is X factor and may be a weak hemolysis?
H. ducreyi
Which haemophilus has a school of fish cell arrangement and has a chancroid or soft chancre?
H. ducreyi
Which haemophilus causes Meningitis, Epiglottitis, Otitis media?
H. influenzae
Which serogroup belongs to the following:
S. dysenteriae
S. flexneri
S. boydii
S. sonnei
A
B
C
D
What MO's are Voges Proskauer (VP) positive?
Klebsella
Enterobacter
Serratia
What MO's are Phenylalanine Positive?
Proteus
Providencia
Morganella
What MO's are VP and Phenylalanine Negative?
E. coli
shigella
Citrobacter
Salmonella
What MO's are lactose Positive?
E. coli
Klebsiella
Enterobacter
Citrobacter (V)
What MO's are H2S positive?
Proteus
Salmonella
Citrobacter (V)
What MO's are Lysine Positive?
Salmonella
E. Coli
What MO's will appear as follows on Media:
EMB Metallic green sheeen
MAC Red/Pink
SS Red/Pink
HE Yellow/orange
XLD Yellow
E. coli
What MO will appear as follows on Media:
EMB Black/blue
MAC Red/Pink
SS Red/Pink
HE yellow/orange
XLD Yellow
Klebsiella and Enterobacter
Klebsiella will have a mucoid appearance
What MO will appear as follows on Media:
EMB Colorless
MAC Colorless
SS Colorless
HE Green
XLD Red
Shigella
What MO will appear as follows on media:
EMB Colorless
MAC Colorless
SS Colorless w/ black center
HE Green w/ black center
XLD Red w/ black center
Salmonella
What MO's will be glucose Fermenter, lactose fermenter and KIA is A/A?
E. coli
Klebsiella
Enterobacter
some citrobacter
What MO's will be Glucose Fermenter, Nonlactose Fermenter with KIA as K/A (Red/Yellow)?
Shigella
S. marcescens
Providencia
Morganella
Y. enterocolitica
Some citrobacter
What MO's will be Glucose Nonfermenter and KIA as K/K (Red/Red)?
Pseudomonas
Acinetobacter
What MO will have a reaction on Lysine iron agar as K/K (purple)?
E. coli
Klebsiella
Serratia
What MO will have K/K with H2S on LIA?
Salmonella
What MO will have R/A on LIA?
Proteus
What MO will have a K/A reaction in LIA?
Shigella
Enterobactor
What MO will have a K/A with H2S in LIA?
Some citrobactor
What is the positive result for ONPG?
Yellow
What reaction does S. marcescens have in TSI?
A/A
What color is the VP positive reaction?
Red
What color is the Citrate positive reaction?
Blue
What color is the Phenylalanine deaminase positive reaction?
Green
In the OF test, what MO gives a Yellow in both closed and open tubes?
E. Coli
In the OF test, What MO gives a yellow in open tube and a green/blue in closed tube?
Pseadomonas
In the OF test, What MO gives a green/blue color in open and closed tube?
Acinetobactor
What MO can infect any body site in immunocompromised patients (CSF/Burn) and causes Swimmer's ear?
Pseudomonas aeruginosa
What MO may resemble Enterobacteriaceae and is oxidase and nitrate reduction =?
Acinetobacter
What MO is sensitive to SXT, oxidizes glucose and maltose?
Stenotrophomonas maltophilia
Flavobacterium?
Often indicates colonization or contamination
Yellow pigment
Most oxidase +
Myrodies odoratum colonies sweet, fruity oder
Moraxella
Usually opportunist
Growth on BA
May grow on MAC
Oxidase +
Will become rod when grown with penicillin
Burkholderia Cepacia
Cystic Fibrosis Pt Cepacia Syndrome (resp tract)
Grows on BCSA or OFPBL Yellow colonies
Weak Oxidase +
Burkholderia pseudomallei
Melioidosis (vietnamese timebomb
Wrinkled colonies
Bipolar staining
Oxidase +
What can grow on MAC, have a metallic sheen, usually beta, smells like grapes or corn taco, and can be mucoid when isolated from Cystic Fibrosis pat?
Pseudomonas aeruginosa
What has pyocyanin production, oxidase positive, growth in 42, oxidizes glucose, TSI: A/no change?
Pseudomonas aeruginosa
What vibrio needs NaCl?
V. alginolyticus
V. parahaemolyticus
V. vulmificus
Which MO do not need NaCl to grow?
V. Cholerae
Aeromonas
Plesiomonas
What causes rice water stool?
V. cholerae
What causes gastric ulcers?
Helicobacter pylori
How do you identify Helicobacter?
Serology
Rapid urease
oxidase
catalase
C. jejuni or C. coli
Usually intestinal
grows on Campy-BAP or skirrow
42 degrees
Cephalothin R
Nalidixic acid S
Hippurate +
C. Fetus
Bactermia in immunocompromised pt
Blood cultures
BAP or Choc
37 degrees
Cephalothin S
Nalidixic Acid R
What motility does Campylobacter have?
Darts
What dz does Legionella cause?
Legionnaires dz
Pontiac Fever
What media does Legionella grow on?
BCYE
What antimicrobic are natural?
Penicilin G and V
What antimicrobic are penicillinase resistant?
Methicillin
oxacillin
nafeillin
What MO is aminopenicillins effective against?
gram negative bacteria
What are the key aspects of natural antimicrobic?
Inactivated by staphylococcal penicillinases
most staphylococci resistant
Pneumococcal resistance due to altered PBP's
What are the key aspects of penicillinase resistant?
Developed to treat infections caused by penicillinase positive staphylococci
staphylococcal resistance now common due to altered pbp
MRS resistant to all beta lactams
What are the aminopenicllins drugs?
Ampicillin
Amoxicillin
What are the carboxypinicillin drugs?
Carbenicillin
Ticarcillin
What are the Ureidopenicillin drugs?
Azlacillin
Mexlocillin
What are the beta lactam and beta lactamase inhibitor's?
Ampicillin/sulbactam
Ticarcillin/clavulanate
What are the narrow spectrum?
cephalothin
cefazolin
What are the extended spectrum?
Cefamandole
Cefaclor
Cefoxitin
What are the broad spectrum?
Ceftizoxime
Ceftriaxone
Cefoperozone
Cefotaxime
Cefepime
Cefoxitin
What are the carbepenem?
Imipenem
What are the monobactam?
Aztreonam
What are known as first generation?
Cephalothin
Cefazolin
What are known as 2 generation?
Cefamandole,
Cefaclor
Cefoxitin
What are the third generation?
Ceftizoxime
Ceftriaxone
Cefoperozone
Cefotaxime
What are the forth generation?
Cefepime