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individual nucleotides are bonded together when phosphate group (on 5' of one nucleotide) reacts with OH group (on 3' end of another) to form a phosphodiester bond.
the chromatin coils again around a _____ protein forming the condensed chromosome seen in mitosis
the mode of DNA replication is termed _____ meaning: two strands of existing DNA each serve as a "template" for new DNA strand, and each new DNA strand bonds to it's "template" forming a complete new molecule of DNA
bonds new nucleotides to the new strand being synthesized. Always adds to the 3' end. Identifies, removes and replaces incorrect bases added.
Polymerase Chain Reaction: method of artificially replicating a specific segment of DNA from a small initial quantity. DNA replication done in "Test Tube" can make millions of copies quickly
thermal cycler, microscopic layers of heated and cooled silicon. these are uesed in ______
copies of DNA to RNA and uses RNA polymerase to amplify RNA. 10 billion copies in 30 minutes
matireals: DNA template, primers, nucleotides, and dna polymerase. Process: 1. denaturation, 2. annealling, 3. extension
In PCR methodology: __________-one complementary strand to each strand (made of DNA not RNA)
In PCR methodology: _________ - DNA template is ________ (2 strands separated) with high heat
In PCR methodology: ________ - Each DNA primer anneals, binding to its complementary sequence on the themplate DNA
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