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The correct order of draw is:

1.Blood cultures
2.Light blue top tubes
3.Serum (red top or red/gray tube)
4.Green tubes
5.Lavender tube
6.Gray top tubes

Sentence mnemonic

BeCause = blood culture
Better = blue top
Specimens = red tops /red and gray
Generate = green top
Perfect = purple
Goals = Gray

Lavender top tube

Contain the (EDTA) inhibit coagulation by binding to calcium present in specimen.

Commont test for Lavender top tube

CBC,includes: RBC, WBC, platelet, hemoglobin, hematocrit, ESR (erythrocyte sed. rate) sickle cell screening.

Light blue top test

Coagulation studies -prothombin time (PT,TT,BT,FDP),(intrinsic path of coagulation.

Prothombin time

Test the ability of the blood to clot.

Bleeding time (BT)

Measures the time required for cessation of bleeding after standardized capillary puncture; focuses on the number of platelets present and their ability to form a plug

Thrombin time (TT)

Determines if adequate fibrinogen is present for normal coagulation.

Green top tubes

Chemistry test (ammonia, carboxihemoglobin,& stat electrolytes)

Gray top tubes

Contain aditives and anti-coagulants (fasting blood sugar,glucose tolerance test) inverted 8 times

Red/gray/tiger top

Inverted 5 times (chemistry test) also called serum separator tube (SST)

Inversion time of tubes

Lavender = 8 times
Light blue = 3 to 4 times
Green = 8 times
Gray = 8 times
Gray/red/tiger 5 times
Yelllow = 8 times

Yellow tube (sterile)

(SPS) cultured for microorganism

Red tubes (plain vacuum tube)

No additive or anti-coagulant (no need to invert the tube after collection- serum chemistry test, serology test, blood bank.


Laboratory test to detect the presence of antibodies, antigens, or immune substances


Radio graphic imaging of the blood vessels.

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