The correct order of draw is:
1.Blood cultures
2.Light blue top tubes
3.Serum (red top or red/gray tube)
4.Green tubes
5.Lavender tube
6.Gray top tubes
Sentence mnemonic
BeCause = blood culture
Better = blue top
Specimens = red tops /red and gray
Generate = green top
Perfect = purple
Goals = Gray
Lavender top tube
Contain the (EDTA) inhibit coagulation by binding to calcium present in specimen.
Commont test for Lavender top tube
CBC,includes: RBC, WBC, platelet, hemoglobin, hematocrit, ESR (erythrocyte sed. rate) sickle cell screening.
Light blue top test
Coagulation studies -prothombin time (PT,TT,BT,FDP),(intrinsic path of coagulation.
Prothombin time
Test the ability of the blood to clot.
Bleeding time (BT)
Measures the time required for cessation of bleeding after standardized capillary puncture; focuses on the number of platelets present and their ability to form a plug
Thrombin time (TT)
Determines if adequate fibrinogen is present for normal coagulation.
Green top tubes
Chemistry test (ammonia, carboxihemoglobin,& stat electrolytes)
Gray top tubes
Contain aditives and anti-coagulants (fasting blood sugar,glucose tolerance test) inverted 8 times
Red/gray/tiger top
Inverted 5 times (chemistry test) also called serum separator tube (SST)
Inversion time of tubes
Lavender = 8 times
Light blue = 3 to 4 times
Green = 8 times
Gray = 8 times
Gray/red/tiger 5 times
Yelllow = 8 times
Yellow tube (sterile)
(SPS) cultured for microorganism
Red tubes (plain vacuum tube)
No additive or anti-coagulant (no need to invert the tube after collection- serum chemistry test, serology test, blood bank.
Laboratory test to detect the presence of antibodies, antigens, or immune substances
Radio graphic imaging of the blood vessels.