11 terms

Ch 20

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1) Words such as did, mom, and pop have something in common compared to the fundamental tool of recombinant DNA technology. In the context of recombinant DNA technology, which term would be used to describe such words?

A) lysogenic
B) prototrophic
C) palindromic
D) conjugation
E) insertion
C
2) Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end sticky?

A) single-stranded complementary tails
B) blunt ends
C) poly-A sequences
D) 5' cap
E) interference
A
3) List two especially useful characteristics of cloning vectors.

A) high copy number and antibiotic resistance gene(s)
B) virulence and lysogenicity
C) ability to integrate into the host chromosome and then causing a lytic cycle
D) nonautonomous replication and transposition
E) reverse transcriptase and ligase activities
A
4) Some vectors such as pUC18 and others of the pUC series contain a large number of restriction enzyme sites clustered in one region. Which term is given to this advantageous arrangement of restriction sites?

A) palindrome
B) consensus sequence
C) multiple cloning site
D) β-galactosidase
E) complementation
C
5) One of the primary reasons for generating a large number of clones in a eukaryotic genomic library is that ________.

A) each cosmid replicates in coordination with the host chromosome
B) lysogenic phages continue to integrate their DNA into the host chromosome, thus reducing the number of desired recombinant clones
C) each vector can take up only a relatively small fraction of the eukaryotic DNA
D) each ligation product is sequence specific
E) the host range of the vector is limited
C
6) In the context of molecular genetics, reverse transcription PCR (RT-PCR) refers to ________.

A) assembling a DNA sequence from an mRNA
B) assembling an RNA sequence from a DNA sequence
C) translating in the 3' to 5' direction
D) transcribing first, then translating
E) making an amino acid sequence from a DNA sequence
A
7) Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot, one generally ________.

A) hybridizes filter-bound DNA with a DNA probe
B) hybridizes filter-bound RNA with a DNA probe
C) examines amino acid substitutions with radioactive probes
D) cleaves RNA with restriction endonucleases
E) ligates DNA with DNA ligase
A
8) Assume that a plasmid (circular) is 3200 base pairs in length and has restriction sites at the following locations: 400, 700, 1400, 2600. Give the expected sizes of the restriction fragments following complete digestion.

A) 400, 800, 1000 (2 of these)
B) 400, 1200, 1600
C) 300, 700, 2200
D) 700, 400, 1400, 2600
E) 300, 700, 1000, 1200
E
9) In which of the following biochemical reactions is it common to use ddNTPs (dideoxyribonucleoside triphosphates)?

A) citric acid cycle
B) DNA sequencing
C) restriction digestion
D) electron transport
E) plasmolysis
B
10) A ddNTP, used often in DNA sequencing, lacks a(n) ________ at the ________ and ________ carbons.

A) OH; 2'; 3'
B) methyl; 2'; 3'
C) carboxyl; 5'; 3'
D) OH; 2'; 5'
E) None of the above is correct.
A
11) The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95°C. Why would such a heat-stable polymerase be beneficial in PCR?

A) Each cycle includes a "hot" saturation phase (95°C), which allows the primers to
anneal to the target DNA.
B) Each cycle includes a "hot" denaturation phase (95°C), which serves to sterilize the
culture.
C) Each cycle includes a "hot" denaturation phase (95°C), which activates the Taq
polymerase.
D) Each cycle includes a "hot" denaturation phase (95°C), which separates the hydrogen
bonds that hold the strands of the template DNA together.
E) More than one of the above are correct.
D