20 terms

Micro 9

Which of the following is an example of a cloning vector?
Recombinant DNA technology is used for all of the following except ________.
culturing unknown organisms
The process of making multiple copies of a DNA molecule is referred to as ________.
Which of the following is not an advantage of obtaining the protein product human growth hormone by recombinant DNA technology rather than extraction from cadavers?
production of endotoxins
Which of these answers is true for restriction enzymes?
Restriction enzymes are useful in genetic engineering when they make staggered cuts in DNA
When two DNA pieces cut with the same restriction enzyme are combined, sticky ends will ________.
associate by complementary base pairing and hydrogen bonds
If DNA ligase was not used in the creation of a recombinant plasmid, ________.
base-pairing would occur but the sugar phosphate backbone would not be connected
A good cloning vector ________.
should have a gene or genes that allows for selection of transformed host cells
All of the following are true of the polymerase chain reaction (PCR) except:
Large amounts of DNA must be isolated from the source organism
Which of the following is not a purpose of genetic modification?
Remove antibiotic resistant plasmids from bacteria
Recombinant DNA can be introduced into a host cell by any of the following methods except ________.
polymerase chain reaction
For the introduction of a genetically modified plasmid into E. coli, ________.
calcium chloride and heat shock can be used
Which of the following statements correctly differentiates a genomic library from a cDNA library?
A genomic library contains fragments of the entire DNA in an organism's genome. A cDNA library contains the coding sequences of eukaryotic genes (minus the introns).
During the Southern blotting technique, what is the purpose of transferring the DNA fragments from the gel to a nitrocellulose filter?
This step attaches the DNA fragments to a permanent substrate, which then can be probed
An ampicillin-sensitive culture of E. coli is transformed with a plasmid that contains the gene of interest plus an ampicillin-resistant gene. If it is then plated on an ampicillin-containing growth medium
only the bacteria with the plasmid will grow
Which of the following might specifically be used as part of a reverse genetics approach to studying a gene?
RNA interference
In the blue-white screening procedure, bacteria that are transformed with recombinant plasmid and cultured in media containing ampicillin and X-gal will ________.
produce white colonies
Which of the following is not a step in Southern blotting?
addition of heat-stable DNA polymerase
Genetic technology has enabled screening for a variety of genetic conditions, and use of this technology is becoming more widely available. Which of the following is likely to become an important issue that will need to be addressed?
the need for legislation to protect the privacy of individuals' genetic information
For Agrobacterium tumefaciens to be used to introduce foreign DNA into a plant cell, that DNA must first be ________.
inserted into the T-DNA region of the Ti plasmid of A. tumefaciens