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48 terms

micro lab final study 1

STUDY
PLAY
black
starch & iodine
red
phenol red in neutral
red
phenol red in 7+
yellow
phenol red in acid
yellow
phenol red in -7
MR
test for acid
yellow
methyl red in neutral
yellow
methyl red negative result
red
methyl red in acid
red
methyl red positive result
VP
tests for acetoin
red
VP test positive
negative
VP test with no color change
OF medium
HIGH concentration of carbohydrate and LOW concentration of peptone
bromothymol blue
indicator in OF medium
yellow
bromothymol blue in the presence of acid
yellow
bromothymol blue, carbohydrate has been catabolized
blue
bromothymol blue, ammonia production
50
nutrient gelatin dissolves at
20
nutrient gelatin solidifies at
gelatinase
nutrient gelatin does not solidify even when cooled, after being hydrolized with this
urease
liberates ammonia from urea
phenol red
used to test for presence of urease
MRVP
used to distinguish between organisms that produce large amounts of acid and those who produce neutral product acetoin
urea
waste product of protein digestion
urea agar
contains peptone, glucose, urea, phenol red
urea agar
the ph is 6.8
pink
urea agar turns this color during ammonia production
ammonia
the ph is 8.4 when this is present
ammonia
when amino acid gets deaminated, you get
green
indicates ferric ion
green
positive for phenylamine deamination
nessler
reagent, indicating ammonia
yellow
in nesslers reagent, indicates ammonia
MIO
motility indole ornithine media
ornithine
tests for carboxylation
purple
positive ornithine test
yellow
negative ornithine test
carboxylation
removal of co2 from amino
positive
ornithine test results if the ph has shifted to basic
indole
production of this indicates a breakdown of tryptophan
kovac's
reagent used in indole test
pink ring
indicator of indole production
motility
large amount of spread away from the stab indicates
black
precipitate is formed when h2s is produced
peptone iron agar deep
medium in sulfur reduction
sulfhydrase
break down of sulfur
halo
if the bacteria uses starch, this is the indicator