Put the steps of the process of using transgenic bacteria to use human insulin in the correct order.
a. DNA ligase seals sticky ends (seals sugar-phosphate backbone of recombinant plasmid)
b. restriction enzyme is used to cut out the human insulin gene and to cut open plasmid
c. sticky ends are attracted to one another, nitrogen bases of sticky ends form H bonds
d. isolate the insulin
e. asexual reproduction
f. transformation occurs