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Enzyme Data Analysis Review
Terms in this set (9)
What is an enzyme?
A protein that speeds up chemical reactions.
What is a catalase?
An enzyme that breaks hydrogen peroxide into water and oxygen.
What is an active site?
The area in which the substrate molecules bind and undergo a chemical reaction
What is a substrate?
A substance which fits into the active site of an enzyme, and is acted upon by the enzyme
What is the equation for the break down of hydrogen peroxide?
Describe the relationship between catalase and hydrogen peroxide. Indicate which is the enzyme, which is the substrate, and what occurs during the reaction.
Catalase breaks down hydrogen peroxide into water and oxygen. The enzyme is the catalase within the liver homogenate. The substrate was the hydrogen peroxide. During the reaction, the catalase in the liver binds with the hydrogen peroxide. The catalse breaks the hydrogen peroxide into water and oxygen, which caused the bubbles to form.
How does temperature and pH affect the reaction rate of the catalase? Propose a way to refine your experiment to find the exact, or OPTIMAL pH and temperature of the catalase.
There is a particular range under which an enzyme which function at its best. If it is too hot or too cold, the enzyme will become denatured. If it is too acidic or too basic, the enzyme will also become denatured. To alter the experiment, we would repeat the procedure with many different temperatures and pHs. We would graph the results to find the point at which the enzyme worked most efficiently.
In 2-3 sentences, summarize the data and information displayed in the graph.
Without the enzyme, a large amount of activation energy is needed. The enzyme lowers the activation energy necessary to complete the reaction.
Lactaid is a product designed to help people who cannot digest milk sugar (lactose) because they are missing the enzyme lactase. Many people are lactose-intolerant, a condition that is mainly genetic. Lactase breaks down lactose into two subunits: glucose and galactose.
To test for the presence of monosaccharides and reducing disaccharide sugars in food, the food sample is dissolved in water, and a small amount of Benedict's reagent is added. The solution should progress in the colors of blue (with no glucose present), green, yellow, orange, red, and then brick red or brown (with high glucose present).
Design an experiment where you would determine how quicly lactaid works to break down milk sugar. Be specific in your description, use drawings if necessary.
1. Gather 11 clear cups.
2. Fill each cup with 5 ounces of whole milk.
3. Take the first cup and add a known quantity of lactaid.
4. As a control, take another cup and do not add any lactaid.
5. After one minute, pour a known quantity of Benedicts into the cups of milk.
6. Record the color (blue is the least amount of glucose progressing to green, then yellow, orange, red, and brick red/brown).
7. Take another cup of milk.
8. Add the lactaid and wait 5 minutes.
9. Add the Benedicts.
10. Record the color.
11. Repeat steps 7-10 adding 5 minutes each time until there are no cups left (15 minutes, 20, 25, 30, 35, 40, 45).
12. Graph the data and form conclusions.
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