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5 Written questions

5 Matching questions

  1. structure of tRNA
  2. DNA replication in prokaryotes
  3. "beads on a string"
  4. Meselson & Stahl Experiment (1959)
  5. histone octamer (4)
  1. a tested the three models of DNA replication by this: bacteria grown in N15; transferred to medium containing 14N. different N-isotope distinguishable
    -prove semi-conservative replication
  2. b -has a primary, secondary (cloverleaf) and tertiary(L-shaped) structyre
    -bulk of it is double stranded bc of distal complementary base(nucleotide) pairs
    -unique anticodons to complement mRNA(5' end of each) and amino acid(3'-adenine by aaRS)
  3. c -all DNA in single circular chromosome
    -proceeds in both directions from ori (origin sequence) to T (termination site)
  4. d many nucleosomes on DNA strand
  5. e -2 copies each of core histones H2A, H2B, H3, H4
    "the core particle" (doesn't include H1)

5 Multiple choice questions

  1. -pre-RNA spliced in multiple ways to produce different mRNAs to encode diff. protein sequences
  2. chromatin loops are attached to nuclear matrix
  3. 1-nuclease digests linker DNA
    2-dissociation with high salt concentration
  4. core particles connected by this
    DNA wrapped +linker=200 bp
  5. section of mRNA before the start codon and after stop codon (not translated)
    -mRNA stability, localization and translation

5 True/False questions

  1. Kozak boxremoves RNA which is then filled in with DNA nucleotides

          

  2. termination of protein synthesis1: class I release factors enter A site
    2: ester bond linking polypeltide chain to tRNA hydrolyzed and completed polypeptide released
    3:release free tRNA from P site, dissociate mRNA from ribosome, disassemble ribosome into large and small subunit.

          

  3. 3' poly (A) tail functions (3)promotes export from nucleus
    promotes translation
    protects mRNA from degradation

          

  4. DNA helicaseunwinds DNA at replication fork with energy from ATP hydrolysis.

          

  5. rRNAmost actively transcribed RNA in cell
    -composes <80% total RNA content
    -highly amplified on 5 human chromosomes containing 100s of genes