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5 Written questions

5 Matching questions

  1. Post-transcriptional modifications (3)
  2. termination of protein synthesis
  3. Shine Dalgarno sequence
  4. pre-Initiation of Protein Syntheisis
  5. coding regions (CDS)
  1. a ribosome identifies start codon using this in prokaryotes
  2. b -process for converting pre-RNA to final RNA
    1: 5' capping (methylated guanine)
    2: 3' polyadenylation
    3: RNA splicing
  3. c 1: class I release factors enter A site
    2: ester bond linking polypeltide chain to tRNA hydrolyzed and completed polypeptide released
    3:release free tRNA from P site, dissociate mRNA from ribosome, disassemble ribosome into large and small subunit.
  4. d -codons decoded and translated to protein
    -begin with start codon, end at stop codon
  5. e 1-codon AUG near 5' end of mRNA
    2-aminoacyl-tRNA binds to P site with aa methionine

5 Multiple choice questions

  1. -semi conservative(parent chain separate and daughter chain base pair with parent chains forming 2 new double helices)
    -antiparallel(5'->3' on both but in opposite directions to each other)
  2. -replicon organization along chromosomes
    -replication timing during S-phase
    -initiation of DNA replication during S-phase
    -organization of replication sites in cell nucleus
  3. -2 copies each of core histones H2A, H2B, H3, H4
    "the core particle" (doesn't include H1)
  4. 1: bring ribosomal subunit to initiation codon(codon AUG near 5' end of mRNA)
    2: bring 1st aa tRNA into ribosome (methionine-tRNA enters P site ;binds to AUG codon and IF2 )
    -IF3 and IF1 released
    3: assemble complete initiation complex(large subunit joins complex; IF2 hydrolyzed through GTP hydrolysis, releasing IF2-GDP)
  5. composed of many splicing factors
    -mediate splicing process

5 True/False questions

  1. 4 distinct ribosomal RNAs-3 in large (60S)subunit(28S, 5.8S, 5S);
    -1 in small (40S)subunit(18S)
    -40S+60S make 80S ribosome (there is no typo lol)

          

  2. ribosomeribosomes transcribing same piece of mRNA creating many copies of same protein
    -5' end codes for N-terminal amino acid
    -3' end codes for C-terminal amino acid

          

  3. bidirectional replication-semi conservative(parent chain separate and daughter chain base pair with parent chains forming 2 new double helices)
    -antiparallel(5'->3' on both but in opposite directions to each other)

          

  4. "linker DNA"many nucleosomes on DNA strand

          

  5. RNA primaseseals okazaki fragments on lagging strand.