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5 Written questions

5 Matching questions

  1. fibrillar centers (fc)
  2. histone octamer (4)
  3. DNA helicase
  4. 5' capping
  5. Shine Dalgarno sequence
  1. a -rDNA genes concentrated here
    -numerous of these compose the nucleolus
  2. b unwinds DNA at replication fork with energy from ATP hydrolysis.
  3. c add 7-methylguanosine (m7G) to 5' end of RNA
    -immediately after initiation
    -attaches to 5' end ot tRNA
  4. d -2 copies each of core histones H2A, H2B, H3, H4
    "the core particle" (doesn't include H1)
  5. e ribosome identifies start codon using this in prokaryotes

5 Multiple choice questions

  1. -pre-RNA spliced in multiple ways to produce different mRNAs to encode diff. protein sequences
  2. ribosomes transcribing same piece of mRNA creating many copies of same protein
    -5' end codes for N-terminal amino acid
    -3' end codes for C-terminal amino acid
  3. Represent DNA packing ration of 10,000:1
  4. 30 nm fiber after 10 nm nucleosome filament coils; helical structure
  5. -anti-codons that bind to codon on mRNA
    -2nd site binds corresponding amino acid in high energy aminoacyl bond

5 True/False questions

  1. nucleosome-lowest level of chromosome organization
    -form fundamental repeating units of eukaryotic chromatin
    -146bp of DNA wrapped almost twice around histone octomer
    -10 nm fibers


  2. okazaki fragmentsinitiates DNA replication on both strands by adding RNA primers
    -1 for the leading strand, 1 for each Okazaki fragment


  3. nucleolar organizer regions (NOR)region of chromosomes where rRNA genes are amplified (>100 copies)


  4. snRNP and splicesome complexes1)RNA primer synthesis by RNA primase
    2)elongation by DNA polymerase III
    3)primer removal and gap filling by DNA polymerase I


  5. chromatin organization on nuclear matrixchromatin loops are attached to nuclear matrix


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