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5 Written questions

5 Matching questions

  1. steps to eukaryotic transcription of pre-mRNA(4)
  2. granular component (gc)
  3. aminoacyl-tRNA synthetase enzyme (aaRS)
  4. Kozak box
  5. activating amino acid as amion-acyl group
  1. a ribosome identifies start codon using this in eukaryotes
  2. b 1)pre-initiation- initiation complex assembles specific sequences at promoter site.
    2)initiation- initiated by pol II following phosphorylation of serine #2 on CTD (C-terminal domain) hepa(7)peptide repeat (52X) on large subunit of RNA pol II
    3)elongation -activated following phosphorylation of serine #5 on CTD ... and involves additional elongation factors
    4)termination-newly synthesized pre-mRNA released from DNA template by action of terminal factors at 3' end of gene.
  3. c uses energy from hydrolysis of ATP (ATP ->ADP+Pi)
  4. d -processing of pre-rRNP particles occurs here
    -mature ribosomal subunits released for transport to cytoplasm
  5. e attach amino acids to tRNAs by high energy amino acyl bonds

5 Multiple choice questions

  1. ribosome identifies start codon using this in prokaryotes
  2. 1-nuclease digests linker DNA
    2-dissociation with high salt concentration
  3. -all DNA in single circular chromosome
    -proceeds in both directions from ori (origin sequence) to T (termination site)
  4. 1: aminoacyl-tRNA selection
    -2nd aminoacyl-tRNA enters A site
    -1st combining with protein elongation factor bound to GTP (EF-Tu-GTP)
    -GTP hydrolyzed and Tu-GDP complex released, leaving aa-tRNA bound in A site
    2: peptide bond formation between the 2 tRNAs (enzyme peptidyl transferase catalyst)
    -forms dipeptidym-tRNA
    3: translocation: ribosome moves 1 codon along mRNA (in 5'-3' direction)
    -deacylated tRNA moves from P site to E site
    -GTP hydrolyzed and EF-G-GDP leaves ribosome
    4:release deacylated tRNA: new aminoacyl-tRNA enters A site
  5. factory for rRNA synthesis and assembly with proteins into pre-ribosomal subunits

5 True/False questions

  1. key components of pre-initiation complex (2)composed of many splicing factors
    -mediate splicing process


  2. DNA replicationunwinds DNA at replication fork with energy from ATP hydrolysis.


  3. termination of protein synthesis1: class I release factors enter A site
    2: ester bond linking polypeltide chain to tRNA hydrolyzed and completed polypeptide released
    3:release free tRNA from P site, dissociate mRNA from ribosome, disassemble ribosome into large and small subunit.


  4. rRNAcentral component of ribosome's protein manufacturing machinery


  5. promoter siteribosomes transcribing same piece of mRNA creating many copies of same protein
    -5' end codes for N-terminal amino acid
    -3' end codes for C-terminal amino acid