Gram + retain the purple coloring of the CV after the decolorizing, while the Gram - do not but appear but turn pink from the Fuchsin.
How are the results of an Acid-Fast Staing read?
Acid-fast microbes,such and members of the genera Mycobacterium and Nocardia, retain carbolfuchsin after acid-alcohol decolorization and appear red; non acid-fast microbes take up the methylene blue counterstain and appear blue.
What is the purpose of Negative Staining?
It is used to make microbial capsules visible. The endospore stain and the flagella stain are special stains that color only certain parts of the bacteria.