tests for micro

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Crystal violet agar
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Terms in this set (25)
Crystal violet agar
selective for gram negative and inhibits gram positive
7.5% sodium chloride agar
inhibitory for all bacteria expect for members of staphylococcus and salt loving bacteria
Mannitol salt agar
inhibitory for all bacteria except for staphylococci
MacConkey
selects for gram negative and indicates lactose fermentation but turning red ex E.coli is bright red
Eosin-methylene blue agar (Levine)
differentiates between enteric lactose fermentors and non fermentors, and identifienes colon bacillus which turn blue-black. fermentors turn pink and non fermentors are clear
blood agar
indicates cultivativation of streptococcus; demonstrates hemolytic properties
Alpha hemolysis- green halo
Beta hemolysis- clear zone around the colonies
gamme hemolysis- no change
pheynlethyl alcohol agar
selects for gram positive
uv radiation kills all cells but.....
B. Subtilits because it has an enzyme to correct the thymine dimers
Oxygen requirement test
aerobes that require oxygen like B.Subtilis would show what
it would show growth at the top of the test tube
oxygen requirement test
facultative anaerobes that don't require oxygen would show....
growth throughout/most near the surface
aerotolerant anaerobes (don't use oxygen, but tolerate it) would show.......growth evenly throughout the test tubemicroaerophiles (have reduced oxygen tension)show growtn just under the surfacecatalase test tests for the enzyme catalase what would a positive test look likebubblesCultivation of anaerobes what would a positive test for anaerobes look likepink in the test tubeWhat would an acid-stain cell look like?pink non are bluewhat two diseases does acid-fast staining indicatesTB and leprosyinterpret the TSI test results all red all yellow red slant and yellow buttnone are fermented all 3 are fermented glucose only is fermentedin milk agar a clear zone surrounding indicates......protease called caseinasestarch agar has a clear zone of hydrolysis shows presence of ______ and adding iodine and seeing a clearing indicates presence of _____-amylase maltosewhat sugar does the cap color correspond with (solution is red at the start) (yellow if it ferments that sugar) green cap white cap red capglucose sucrose lactosethe addition of a kovack's reagent and it turning red indicates what?tryptophan being hydrolizedB-methyl red tests detects the presence of......large acid products E.coli(pH = 4/red) is more red than E.aerogenus (pH=6/yellow)What color does the tube turn if H2S was produced?blackwhen reading a Citrate test what does a blue coloration tell youthat citrate is used as her sole carbon sourceinterpret these nitrate reduction results A + B = red A + B + zinc = clear A + B + zine = rednitrate was reduced nitrate was reduced completely nitrate was not reduced