First visualisation of microdomains!!!!!
FRET based assay. Chromophore tagged catalytic and regulatory subunits of PKA. Dissociate and FRET signal lost when cAMP binds.Hypothesised that cAMP microdomains might occur in T tubules of rat cardiomyocytes (Gs, AKAP-anchored PKA, ACs found throughout tubules).
Upon stimulation of β-ARs, saw a compartmentalised rise in cAMP in T-tubules.