Protein Electrophoresis Lab
by Rob Swatski, Associate Professor of Biology at HACC-York Campus (HACC, Central Pennsylvania's Community College) http://robswatski.virb.com/
Terms in this set (22)
The process where macromolecules are separated according to size using electrical charges is called _____.
The size of a molecule is called its _____.
A negatively charged molecule will move at a specific rate toward an electrode having a _____ charge.
What was the gel that we used in lab made of?
We had to make sure that all of the proteins used in lab had a _____ charge.
The _____ of the gel are placed on the negative side of the chamber.
All proteins are called _____, and are made of chains of amino acids.
The pH at which a compound is electrically neutral is called the _____ point.
The electrophoresis _____ ensures that the pH of the solution around the gel remains constant.
Name the chemical that breaks the disulfide bones of the proteins, causing them to open up and become denatured.
Name the chemical that coats the amino acids of the proteins and masks their original charges.
The gel we used in lab is not a solid body, it is filled with tiny spaces called _____.
The larger a protein is, the _____ it moves across the gel.
The smaller a protein is, the _____ it moves across the gel.
The standard proteins that we used during lab are stained with different colors called _____ markers.
The _____ was added to the unknown protein samples to help let us know when to stop electrophoresis.
There is a(n) _____ relationship between the size of the protein and the distance it migrates on the gel.
The molecular weight of a protein is measured in units called _____.
What type of graph paper did we use to plot the protein's migration distance by its molecular weight?
We heated the protein samples at 100 degrees Celsius for 3 minutes at the beginning of the experiment in order to _____ the proteins.
Name this lab tool.
What kind of chemical is this?
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