23 terms

Micro Lab 1- Microscope/Staining Review

What is the function of staining in microscopy
to increase the contrast of the specimen AND to assist in classification of the specimen
For which size of specimen would a microscope be required?
Which of the following statements is true regarding electron microscopy?
Stains are used to enhance contrast during electron microscopy.
Light microscopy can view speciman
1mm - 200nm
Most common form of light microscopy is
The specimen to be observed rests on ____________.
What is the role of the condenser lens?
to focus the light to a high intensity in a small area
Which of the following does the bulk of the magnification of the specimen?
What type of image would you see with a transmission electron microscope?
a two-dimensional image of ribosomes
Which wavelength would result in the highest resolution?
0.01 nm
The condenser lens of an electron microscope _____________________.
is an electromagnet
Which of the following are characteristics shared by both light microscopes and electron microscopes?
condenser and objective lens
3 main categories of stains used by Microbiologist
Simple, Differential, Special
Crystal violet purple stain
stains most bacteria uniformly
safranin a red dye
stains most bacteria pink or red
methylene blue
stains most bacteria light blue
most stains used in Microbiology are
differential stains
Gram stain is most frequently used to _____
determine between Gram positive and Gram negative
Gram positive has a _____
thick outter cell wall composed of peptydoglycan
Gram negative has a _____
thin outter cell wall
Gram staining requires 7 steps
Crystal violet, rinse, mordant, rinse, decolorizing agent, counterstain safarnin, and rinse
Gram positive
stained a deep purple
Gram negative
stained pink by safranin