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Micro Ch. 4 Quiz Microscopy, Staining, and Classification

Microscopy, Staining, and Classification Blackboard Quiz
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confocal
Which of the following types of microscope is being used to examine the relationships between microbes in biofilms on stromatolites?
A. scanning electron
B. transmission electron
C. confocal
D. scanning tunneling
2000X
The limit of useful magnification for a light microscope is ____________________.
A. 1000X.
B. 2000X.
C. 100X.
D. 10,000X.
It attaches cells firmly to the slide's surface
What is the purpose of fixation in smear preparation?
A. It keeps cells from drying out during staining.
B. It attaches cells firmly to the slide's surface.
C. It enables cells to take up more stain.
D. It temporarily keeps cells from growing.
biochemical tests
If you wanted to determine if a microorganism fermented a particular carbohydrate, which of the following kinds of tests would you use?
A. biochemical tests
B. phage typing
C. serology
D. morphology
Ethanol/acetone
In the Gram stain, which of the reagents actually differentiates between Gram positive and Gram negative cells?
A. Iodine
B. Ethanol/acetone
C. Safranin
D. Crystal violet
Large numerical aperture lens, short wavelength radiation
Which of the following combinations would give the best microscopic resolution?
A. Small numerical aperture lens, long wavelength radiation
B. Small numerical aperture lens, short wavelength radiation
C. Large numerical aperture lens, long wavelength radiation
D. Large numerical aperture lens, short wavelength radiation
an electron beam
Which of the following has the shortest wavelength?
A. violet light
B. an electron beam
C. red light
D. white light
IV, II, I, III, V
Put the following units of measurement in order, from largest to smallest:
I. Millimeter
II. Centimeter
III. Micrometer
IV. Meter
V. Nanometer

A. V, III, I, II, IV
B. IV, II, I, III, V
C. V, III, II, I, IV
D. III, V, I, II, IV
the phase plate
All of the following are components of a bright-field compound microscope EXCEPT:
A. the oil immersion objective lens.
B. the phase plate.
C. the condenser lens.
D. the scanning objective lens.
Basic dyes are attracted to the negatively-charged surface of cells
Which of the following is a true statement concerning basic dyes?
A. Eosin is an example of a basic dye.
B. Basic dyes work best at a low pH.
C. Basic dyes are also known as anionic chromophores.
D. Basic dyes are attracted to the negatively-charged surface of cells
Schaeffer-Fulton
Which of the following staining procedures is useful for visualizing endospores?
A. Ziehl-Neelsen
B. Gram
C. Osmium tetraoxide
D. Schaeffer-Fulton
serology
The designation E. coli O157:H7 is a result of which type of microbial identification process?
A. biochemical tests
B. serology
C. phage typing
D. morphology
an electron microscope
All of the following types of microscope can be used to view living specimens EXCEPT ____________________.
A. an atomic force microscope.
B. a dark-field microscope.
C. an electron microscope.
D. a bright-field microscope
Each taxon includes all of the taxa above it
All of the following statements concerning taxonomy are true EXCEPT:
A. Species of asexual organisms are defined as collections of strains.
B. All taxa have Latin names.
C. Each taxon includes all of the taxa above it.
D. The genus name and the specific epithet together are called a binomial
the kingdom
The domain, a taxon proposed by Carl Woese, contains or replaces which of the following taxonomic groups?
A. the genus
B. the phylum
C. the kingdom
D. the order
Answers A, B, and C are correct
In microscopy, which of the following plays an important role in visualizing extremely small objects clearly?
A. wavelength of radiation used
B. numerical aperture of the lens
C. contrast between the specimen and its background
D. Both A and C are correct.
E. Answers A, B, and C are correct
Acid-fast stain
Which of the following stains is used for visualizing Mycobacterium?
A. Negative stain
B. Endospore stain
C. Gram stain
D. Acid-fast stain
Scanning electron micrographs typically appear two-dimensional
All of the following are aspects of scanning electron microscopy EXCEPT:
A. Whole specimens can be observed.
B. Specimens must be placed into a vacuum.
C. Scanning electron micrographs typically appear two-dimensional.
D. Secondary electrons are used to visualize the specimen
subspecies
Which of the following taxonomic groupings was NOT part of the traditional Linnaean system of classification?
A. family
B. subspecies
C. class
D. genus
primary stain
In the Gram stain, crystal violet serves as the ____________________.
A. primary stain.
B. mordant.
C. decolorizing agent.
D. counterstain