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Bacterial Transformation Lab

Terms in this set (16)

What is Genetic Transformation?
Change caused by genes, and involves the insertion of gene into an organism to change the organism's trait.
What did we insert into the bacteria to get it to glow under ultraviolent light?
We used gene form a jellyfish that codes for Green FLuourescent Protein (GFP)
What did we use to move the genes into the bacteria?
We used a plasmid. Plasmids contain one or more traits that help with the bacterial survival.
What organism would be the best choice for a Genetic Transformation?
A bacterium because they are small single-celled organisms that would be able to reproduce quickly.
What is the goal of Genetic Transformation?
To change an organism's phenotype.
What materials did we use to determine if E. coli is affected by ampicillin?
2 LB/agar plates, E. coli, and ampicillin
What does bacteria contain?
plasmids
How is a bacteria resistant to an antibiotic?
The plasmid contains the GFP gene and a gene codes for protein that give the bacteria resistance to ampicillin.
What chemical did we use in the transformation solution?
Calcium Chloride
How did you see whether the bacteria is resistant to ampicillin?
You would compare the -pGLO LB/amp and the +pGLO LB/amp to see which one kept reproducing.
Why do you need a control plate?
A control plate helps you find and understand your results from your experiment.
What original traits did not change in this experiment?
color of the bacteria
size of colonies
shape of colonies
What new traits changed in this experiment?
Ampicillin resistance
fluorescent
What do you need to see the green color in the bacteria?
You need arabinose in the agarose plate and the UV light to be present in the bacteria's environment.
What does arabinose and the UV light do to show the green color?
Arabinose causes the GCF trait to arise, and the UV light resonates it.
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