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Genetic change in bacteria can be brought about by
mutation conjugation transduction transformation.
An enzyme that catalyzes the cutting and resealing of DNA, and is translated from insertion sequences, is
What are the products of semiconservative replication for a double-stranded DNA molecule?
Two double-stranded DNA molecules, each consisting of one parental strand and one daughter strand.
What is the function of the parental DNA in replication?
It serves as the template for DNA replication.
What is considered to be the average natural mutation rate that occurs during DNA replication?
One in every billion nucleotides replicated.
A mutation that affects the genotype of the organism but not the phenotype is called a
A base substitution that changes a codon coding for an amino acid to a stop codon is called a
Bacterial conjugation is often referred to as bacterial sex. Why is this term inaccurate?
Conjugation does not result in the formation of new offspring.
What must occur for bacterial conjugation to take place?
The cells must come into contact with each other.
Competent cells are cells that
can take up DNA from their surrounding environment and integrate it into their own chromosomes by recombination.
Mice that are injected with only the R strain of Streptococcus pneumoniae
stay healthy, because their immune systems can kill this strain easily.
What characteristic of the S strain allows it to evade the immune system of the mice?
The cells have a capsule.
What most likely explains the recovery of live S strain cells from a mouse injected with heat-killed S strain mixed with live R strain cells?
The R strain picked up the S strain DNA, enabling it to produce a capsule
Which finding is most surprising from Griffith's experiments?
S strain cells are isolated from the blood of mice infected with heat-killed S strains and live R strains.
What is unique about transduction compared to normal bacteriophage infection?
Transduction transfers DNA from the chromosome of one cell to another.
How is generalized transduction different from specialized transduction?
Generalized transduction is initiated during lytic cycle of a virulent bacteriophage; specialized transduction is initiated during the lysogenic cycle of a temperate bacteriophage.
When a transducing phage interacts with a new host cell,
the DNA from the previous host can recombine with the new host chromosome.
What is the outstanding characteristic of the Kingdom Fungi?
Members absorb dissolved organic matter.
Which of the following characterizes the Domain Bacteria?
prokaryotic cells; ester linkages in phospholipids
Which of the following statements about members of the Kingdom Plantae is false? They are multicellular They use organic carbon sources.
They undergo photosynthesis They are composed of eukaryotic cells They synthesize organic molecules.
They use organic carbon sources.
Which of the following statements about the members of the Kingdom Animalia is false?
They are multicellular They undergo photosynthesis.
They are composed of eukaryotic cells They ingest nutrients through a mouth They are heterotrophs.
They undergo photosynthesis.
A bacterial species differs from a species of eukaryotic organisms in that a bacterial species
is a population of cells with similar characteristics.
What is a reason for NOT classifying viruses in one of three domains rather than in a fourth domain?
Viruses are not composed of cells.
Which of the following best defines a strain?
a pure culture that is not totally identical to other cultures of the same species
Into which group would you place a photosynthetic organism that lacks a nucleus and has a thin peptidoglycan wall surrounded by an outer membrane?
Proteobacteria (gram-negative bacteria)
How are negative stains different from other types of stains?
They stain the background, leaving the cells colorless.
Following an endospore stain, how does one distinguish endospores from vegetative cells?
Vegetative cells are pink, endospores are green.
What is the fundamental purpose of staining in light microscopy?
To increase the contrast and visibility of the specimen
Which of the following is NOT a characteristic inherent of the non-endospore-forming gram-positive rods?
Staphylococcus and Streptococcus can be easily differentiated in a laboratory by which one of the following?
growth in high salt concentrations
Which of the following is NOT a characteristic of spirochetes?
easily observed with brightfield microscopy
Which of the following pairs is mismatched? dissimilatory sulfate-reducing bacteria - produce H2S// archaea - extremophiles// actinomycetes - reproduce by fragmentation// chemoautotrophic bacteria - fix atmospheric nitrogen// Cytophaga - a gliding, nonfruiting bacterium
chemoautotrophic bacteria - fix atmospheric nitrogen
Which of the following bacteria is gram-positive? Pseudomonas, Salmonella, Streptococcus, Bacteroide Rickettsia
Which of the following statements about the causative agent of Rocky Mountain spotted fever is FALSE? It is an intracellular parasite. It is transmitted by ticks It is in the genus Rickettsia.
It is found in soil and water. It is gram-negative.
It is found in soil and water.
What bacteria are responsible for more infections and more different kinds of infections?
All of the following bacteria are gram-positive. Which does NOT belong with the others? Actinomyces Bacillus Corynebacterium
You have isolated an aerobic gram-positive, endospore-forming bacterium that grows well on nutrient agar. To which of the following groups does it most likely belong?
All of the following bacteria are motile. Which does NOT have flagella? Escherichia, spirochetes
Pseudomonas, helical bacteria, Salmonella
T or F Two distinguishing factors of the epsilonproteobacteria that they are motile and microaerophilic.
Which of the following statements about helminths is FALSE? All are parasites. They are multicellular animals. They have eukaryotic cells. They are heterotrophic. Some have male and female reproductive organs in one animal.
All are parasites.
Which of the following statements regarding fungi is FALSE? Fungi reproduce by forming asexual or sexual spores. Fungi are eukaryotic heterotrophs.
Most fungi are pathogenic for humans. Most fungi grow well in acidic culture condition. Fungi tolerate low moisture conditions.
Most fungi are pathogenic for humans.
Which of the following pairs is mismatched? anamorphs lack spores Ascomycota conidiospores
Zygomycota sporangiospores microsporidia lack mitochondria Basidiomycota basidiospores
anamorphs lack spores
What spores are asexual spores?
Arthroconidium, Blastoconidum, Chlamydoconodium, Condiospore, Sporangiospore
Which of the following statements regarding protozoa is FALSE? Nearly all protozoa cause disease. Protozoa are unicellular eukaryotes.
Most protozoa reproduce asexually. Protozoa are common in water and soil. Some protozoan pathogens are transmitted by arthropod vectors.
Nearly all protozoa cause disease.
Which of the following pairs is mismatched?
teleomorph produces both sexual and asexual spores, coenocytic hyphae hyphae with cross-walls
dimorphic fungus grows as a yeast or a mold, systemic mycosis fungal infection of body organs,
dermatomycosis fungal infection of the skin
coenocytic hyphae hyphae with cross-walls
Which of the following tends to be more complex in a parasitic helminth than in free-living helminths?
Which of the following statements is FALSE? Fungi produce sexual spores. Fungal spores are highly resistant to heat and chemical agents. Fungal spores are used in identification of fungi. Fungi produce asexual spores. Fungal spores are for asexual or sexual reproduction.
Fungal spores are highly resistant to heat and chemical agents.
Which of the following pairs is mismatched? dinoflagellates - paralytic shellfish poisoning
brown algae - algin red algae - agar diatoms - petroleum green algae - prokaryotic
green algae - prokaryotic
All of the following are characteristic of the Platyhelminthes EXCEPT that they
have highly developed digestive and nervous systems.
In the malaria parasite life cycle
humans are the intermediate host and mosquitoes are both the definitive host and the vector.
Three weeks after a river rafting trip, three family members experienced symptoms of coughing, fever, and chest pain. During the rafting trip, the family had consumed crayfish that they caught along the river banks. An examination of the patients sputum revealed helminth eggs, and serum samples were positive for antibodies to Paragonimus. All of the family members recovered following treatment with praziquantel. In the Paragonimus life cycle,
humans are the definitive host and crayfish are the intermediate host.
Assume you have isolated a multicellular heterotrophic organism that produces coenocytic hyphae, motile zoospores, and cellulose cell walls. It is most likely a(n)
You see acid-fast oocysts in a fecal sample from a patient who has diarrhea. What is the MOST likely cause?
True or False Arthropod vectors are blood-sucking animals such as ticks, lice, and fleas that transmit microbial pathogens.
T or F Some species of dinoflagellates produce neurotoxins that cause fish kills and red tides.
T or F The sporozoite, merozoite, gametocyte, and ring stages are all part of the Plasmodium life cycle.
Most cases of hookworm infection are acquired by ingestion of eggs in contaminated food or water.
What is the most significant support for the idea that viruses are nonliving chemicals?
They cannot reproduce themselves outside a host.
Which of the following statements about viral spikes is FALSE? They are found only on nonenveloped viruses. They are used for attachment. They may cause hemagglutination.
They bind to receptors on the host cell surface.
They are composed of carbohydrate-protein complexes
They are found only on nonenveloped viruses. they are founf on enveloped viruses
Which of the following is NOT utilized to culture viruses? laboratory animals/ bacterial cultures/ embryonated eggs/ animal cell cultures/ culture media
Bacteriophages and animal viruses do NOT differ significantly in which one of the following steps? attachment/ biosynthesis/ uncoating/ penetration/
Which of the following statements is FALSE?
A prophage is phage DNA inserted into a bacterial chromosome. The prophage makes the host cell immune to infection by other phages. Prophage genes are repressed by a repressor protein coded for by the prophage. A prophage may result in new properties of the host cell. A prophage can "pop" out of the chromosome.
The prophage makes the host cell immune to infection by other phages.
Lysogeny can result in all of the following EXCEPT
immunity to reinfection by the same phage.
immunity to reinfection by any phage.
acquisition of new characteristics by the host cell.
specialized transduction. phage conversion.
immunity to reinfection by any phage.
Which of the following statements is NOT true of lysogeny? It causes lysis of host cells.
Prophage is inserted into the host genome. Lytic cycle may follow lysogeny. It is a "silent" infection; the virus does not replicate. It can give infected pathogens the genetic information for toxin production.
It causes lysis of host cells.
A virus ability to infect an animal cell depends primarily upon the
presence of receptor sites on the cell membrane.
The most conclusive evidence that viruses cause cancers is provided by
cancer following injection of cell-free filtrates.
Bacteriophage replication differs from animal virus replication because only bacteriophage replication involves
injection of naked nucleic acid into the host cell.
Generally, in an infection caused by a DNA-containing virus, the host animal cell supplies all of the following EXCEPT RNA polymerase.
nucleotides// DNA polymerase// tRNA// None of the answers are correct
Which of the following places these items in the correct order for DNA-virus replication?
2. DNA synthesis
Viruses that utilize reverse transcriptase belong to the virus families
Hepadnaviridae and Retroviridae.
A lytic virus has infected a patient. Which of the following would best describe what is happening inside the patient?
The virus is causing the death of the infected cells in the patient.
Which of the following statements about viruses is FALSE? Viruses contain DNA or RNA but never both.// Viruses contain a protein coat// Viruses use the anabolic machinery of the cell// Viruses have genes// Viruses use their own catabolic enzymes.
Viruses use their own catabolic enzymes.
Some viruses, such as human herpesvirus 1, infect a cell without causing symptoms. These are called
Assume a patient had chickenpox (human herpesvirus 3) as a child. Which line on the graph in the figure would show the number of viruses present in this person as a 60-year-old with shingles (human herpesvirus 3)?
The following steps occur during multiplication of herpesviruses. Which is the third step?
Which one of the following steps does NOT occur during multiplication of a picornavirus? synthesis of + strands of RNA// synthesis of DNA//synthesis of viral proteins// synthesis of - strands of RNA
None of the answers is correct.
synthesis of DNA
Most RNA viruses carry which of the following enzymes? DNA-dependent DNA polymerase//
lysozyme// reverse transcriptase// RNA-dependent RNA polymerase//ATP synthase
RNA-dependent RNA polymerase
T or F Dogs do not get measles because their cells lack the correct receptor sites for that virus.
Helical and icosahedral are terms used to describe the shapes of a virus envelope. T or F
False - Capsid
Viruses are the only known infectious agents that are obligatory intracellular parasites. T or F
Most drugs that interfere with viral multiplication also interfere with host cell function. True or False
Why are archaea and bacteria—both collections of prokaryotic organisms—grouped into different domains while organisms like fungi and animals are in the same domain?
Bacteria and archaea differ significantly in their rRNA sequences, but fungi and animals share some rRNA characteristics.
While working with a broth culture of archaea in the laboratory, you accidentally spill some of the microorganisms on yourself. Do you need to be concerned about developing an infection? Why or why not?
No; archaea are not pathogenic.
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