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46 terms

Microbiology Lab Quiz 1

STUDY
PLAY
What is the purpose of incubating the unopened plates?
To use as a contol group. They will not change so one would have enough to control to study off of.
If growth appears on the unopened plates what probably happend?
it was contaminated between the autoclave and time of incubation.
Why were the specific types of exposure chosen for this exercise?
To procide bateria for the growth and produce a reaction for the exercise
Why were you asked to incubate the blates at two different temperatures?
They will likely be affected differently at different temperatures depending on how the bateria feeds of the media
What is growth media?
nutrients that allow bateria to be isolated and cultured
Why do microbiologists need growth media?
to classify bateria as a prokaryote (no nucleus) we need to give the bacteria what it likes to eat.
What are the requirements of growth media?
-carbon, oxygen, nitrogen, hydrogen
-temp
-ph
-pressure
-osmolity
What are the categories of media?
physical states
chemical composition
categories of media: physical states
liquid
semisolid
solid, can be solidified
solid, cannot be solidified
categories of media: chemical composition
Defined: synthetic, know the componets
Complex: non-synthetic, dont know exact porportions
Growth means in micro:
^ in population no size
Slant tube provides
more surface area in more confined space
Synthetic
organic and inorganic compounds in an exact chemical formula
complex/ non-synthetic
at least 1 ingredient that is not chemically definable.
enriched media
complex organic substances such as blood, serum, hemoglobin, or special growth factors required by fastidious microbes
General Purpose
grows a broad range of usually non-synthetic microbes
6 types of culture media
-defined
-complex
-selective
-differential
-anaerobic
-transport
An exapmple of selective media
same sample but change the Ph one will grow bateria and one will grow fungus.
Beta-Hemolysis
loves blood
Alpha-Hemolysis
ehh it's okay blood
gamma-hemolysis
no blood
most commonly used media (4)
-nutrient broth
-trypticase soy broth agar
-nurtient agar
-brain and heart infusion broth agar
Nutrient Broth
-Liquid beef extract and peptone
Trypticase soy broth or agar
-beef soy extract
Nutrient Agar
-Solid beef extract, peptone, agar
Brain and Heart infusion broth agar
-Beef, heart, brain extract
Agar
-Polysacharide from red algae
Ubiquity
-The theory of being everywhere.
The 5 I's of Culturing Microbes
-Inoculation
-Incubation
-Isolation
-Inspection
-Identification
Inoculation
growing bateria to study
CFU
Colony forming unit
Pure culture
culture composed of cells arising from a single progenitor (CFU)
Incubation
-Temp controlled chamber
-microbe multiplies and produces macroscopically observeable growth
Isolation
-If one bacterial cell is separated and has space on nutrient surface it will grow a colony.
Rhizoid
-Thick Leg Like shape
Fillimentous
-looks like filliments sticking out from middle
Spindle
-oval shape
Undulate
-Shallow curves around the bateria
lobate
-long curves around the bacteria
curled
-lots of circular motions around the bacteria
filiform
-leg like extentions from middle
pulvinate
-slightly higher than just convex
umbonate
- looks like a boob with a nipple
mixed culture
-2 or more species
contaminated culture
-unwanted microbes
Aseptic
-without contamination