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What is the purpose of incubating the unopened plates?
To use as a contol group. They will not change so one would have enough to control to study off of.
If growth appears on the unopened plates what probably happend?
it was contaminated between the autoclave and time of incubation.
Why were the specific types of exposure chosen for this exercise?
To procide bateria for the growth and produce a reaction for the exercise
Why were you asked to incubate the blates at two different temperatures?
They will likely be affected differently at different temperatures depending on how the bateria feeds of the media
Why do microbiologists need growth media?
to classify bateria as a prokaryote (no nucleus) we need to give the bacteria what it likes to eat.
What are the requirements of growth media?
-carbon, oxygen, nitrogen, hydrogen
categories of media: physical states
solid, can be solidified
solid, cannot be solidified
categories of media: chemical composition
Defined: synthetic, know the componets
Complex: non-synthetic, dont know exact porportions
complex organic substances such as blood, serum, hemoglobin, or special growth factors required by fastidious microbes
An exapmple of selective media
same sample but change the Ph one will grow bateria and one will grow fungus.
most commonly used media (4)
-trypticase soy broth agar
-brain and heart infusion broth agar
-Temp controlled chamber
-microbe multiplies and produces macroscopically observeable growth
-If one bacterial cell is separated and has space on nutrient surface it will grow a colony.
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